For purification of archive-quality DNA from a wide variety of sample types

Features

High-molecular-weight DNA in the range of 100–200 kb

Archive-quality DNA isolation for long-term storage

Reproducible DNA purification

A complete solution for sample-to-storage purification

Convenient, scalable purification procedure

Product Details

Gentra Puregene Kits enable purification of high-molecular-weight (100–200 kb) DNA suitable for archiving. The scalable purification procedure gently removes contaminants and inhibitors and allows samples to be purified for use as long-term references.

To improve availability and address demand more efficiently we implemented an in-house manufacturing solution. This leads to product name and catalogue number changes . This Change will not reflect on the quality and performance of the products. The legacy products will be discontinued on 28.02.2022 and be available only until stocks last.

Please use the table in Performance to  find the product you need.

Performance

The purity of DNA has a significant effect on the accuracy of results obtained in downstream applications. Sensitive downstream applications, such as PCR, demand use of DNA of the highest quality and molecular weight for reliable results. Puregene Kits remove contaminants and enzyme inhibitors, enabling purification of highly stable DNA well-suited for archiving. Purified DNA is of high quality demonstrated by molecular weights of 100–200 kb  and performs well in sensitive downstream applications including PCR and restriction digestion.

Catalog number changes in the Purgene line

Existing Name                                               Cat. no.New name                                              New cat. no

Gentra Puregene Blood Kit (120 ml)        158467Puregene Blood Kit (120 ml)                      158023

Gentra Puregene Blood Kit Plus (1000 ml)158489Puregene Blood Kit (1000 ml)                      158026

Gentra Puregene Cell Kit (8 x 108)                158767Puregene Cell Kit (8 x 108)                      158043

Gentra Puregene Cell Kit Plus (6.7 x 109)158788Puregene Cell Kit (6.7 x 109)                      158046

Gentra Puregene Tissue Kit (4 g)                158667Puregene Tissue Kit (4 g)                      158063

Gentra Puregene Tissue Kit (33 g)                158689Puregene Tissue Kit (33 g)                      158066

Gentra Puregene Blood Kit (1000 ml)        158389Alternative: Puregene Blood Kit (1000 ml)   158026

Principle

Cells are lysed with an anionic detergent in the presence of a DNA stabilizer. The DNA stabilizer limits the activity of intracellular DNases and also DNases found elsewhere in the environment. RNA is then removed by treatment with an RNA digesting enzyme. Other contaminants, such as proteins, are removed by salt precipitation. Finally, the genomic DNA is recovered by precipitation with alcohol and dissolved in hydration solution (1 mM EDTA, 10 mM Tris·Cl pH 7.5). Purified DNA typically has an A260/A280 ratio between 1.7 and 1.9 and is up to 200 kb in size. The DNA can be safely stored at 2–8°C, –20°C or –80°C.

Procedure

The simple Puregene procedure uses a modified salting-out precipitation method for purification of DNA . No mixing or dilution of solutions is necessary, and hands-on time is minimized. The procedure provides convenient stopping points that allow safe, temporary storage of partially purified samples.

Applications

DNA purified using Gentra Puregene Kits is highly stable and suited for use in a wide range of applications, such as:

DNA archiving

PCR

SNP analysis

Southern blotting

Supporting data and figures

Archive-quality DNA.

DNA was purified from replicate whole blood samples using the Gentra Puregene Blood Kit. Purified DNA was analyzed by agarose gel electrophoresis after storage for 18 years at 4°C. M1: Uncut Lambda DNA; M2: Lambda HindIII marker.