The LudgerTag V-Tag Glycopeptide Labeling kit is designed for the fluorophore labeling of enzymatically digested peptides and glycopeptides followed by enrichment of the labeled glycopeptides. Labeled glycopeptides can be separated using MALDI-MS and HILIC HPLC or UHPLC chromatography. We recommend the LudgerSep N2 HILIC column (LS-N2-2.0X150) for HPLC or columns such as the Waters BEH Glycan 2mm x 150 mm for UHPLC.

The method provides both glycan identity and quantitation as well as glycan attachment site and site occupancy

Reliable mAb Glycoprofiling – Provides MALDI-MS and UHPLC data comparable to the gold-standard 2-AB and 2-AA methods
Quick and Easy – Labeling and enrichment in 2 hours
Minimal Sample Needed – Use as little as 10 µgs of mAb glycoprotein
Validated for GMP Labs – Validated to ICH Q2(R1) standards and tested in glycoprofiling labs
High-throughput Compatibility – Ready for 96-well based assay automation

Dye Properties: Mass = 434.42 (resulting in a glycopeptide mass increase of 319.33 Da.) FluorescenceL ex = 250 nm, em = 360 nm.

Number of Samples: 24 separate analytical samples per kit.

Amount of Sample: From approximately 1 µg to 50 µg of glycoprotein per sample.

Suitable Samples: IgG single subclass glycoproteins. Other glycoproteins can be used, but note that if there are multiple glycosylation sites, the glycopeptides may need to be separated by C18 HPLC before HILIC analysis. It is recommended that samples do not have amine containing solvents (e.g. Tris type buffers) or reduction/alkylation agents as they will interfere with the V-tag labeling. Removal of the amine containing solvents and reduction/alkylation agents can be accomplished using devices which are designed for desalting and buffer exchange, as well as removal of low-molecular weight compounds

Labeling Selectivity: One V-tag label for every IgG glycopeptide N-terminus.

Storage: Store the V-tag dye at -20˚C in the dark. Protect from sources of heat, light and moisture. Once used, extra dye solution can be frozen and re-used.

Shipping: The product can be shipped at ambient temperature.