LudgerTag 2-AA多糖标记试剂盒(2PB 还原剂)
2-AA (2-aminobenzoic acid) is considered by many to be a superior replacement for 2-AB in most types of complex glycan analysis. 2-AA labeling is reported to have a higher fluorescence and gives higher labelling efficiencies than 2-AB.

Two kits are available which differ in the type of reductant supplied: sodium cyanoborohydride or non-toxic 2-picoline borane.

The 2-AA labeling kit contains two sets of the following reagents (15 samples per kit) supplied in glass ampoules sealed under pure nitrogen:

LT-KAA-A2
2-Aminobenzoic acid (2-AA dye)
Dimethyl sulfoxide (DMSO)
Acetic acid
Sodium cyanoborohydride

LT-KAA-VP24
2-Aminobenzoic acid (2-AA dye)
Dimethyl sulfoxide (DMSO) / Acetic acid solution
2-picoline borane

Number of Samples One 2-AA labeling kit contains reagents to label up to 30 separate analytical samples per kit

Dye purity >99% by HPLC

Molecular weight 137

Lambda-ex 320 nm

Lambda-em 420 nm

Amount of Sample From 25 pmol up to 25 nmol glycans per sample.

Suitable Samples Any purified glycans with free reducing termini can be labeled.

Structural Integrity No detectable (< 2 mole per cent) loss of sialic acid, fucose, sulfate, or phosphate.

Labeling Efficiency Typically > 85 % (dependent on sample).

Labeling Selectivity Essentially stoichiometric labeling.