A1 Glycan Synonyms: A1 N-linked oligosaccharide. G2S1, A2G2S1, A2G(4)2S(6)1.

Description: Monosialylated, bi-antennary complex-type N-glycan (oligosaccharide).

Molecular Weight: 1933

Purity: >90% pure as assessed by a combination of 1 H-NMR and HPLC.

Sources: A1 glycan is found on many mammalian glycoproteins including IgG, gamma globulins, and many serum glycoproteins. This product is typically purified from the oligosaccharide pool released from bovine serum by hydrazinolysis using a combination of HPLC and glycosidase digestion

Form: Dry. Dried by centrifugal evaporation from an aqueous solution. Contains ammonium salt to stabilise against desialylation.

Storage: -20˚C both before and after dissolution. This product is stable for at least 5 years as supplied.

Shipping: The product can be shipped at ambient when dry. After dissolution, ship on dry ice.

Handling: Allow the unopened vial to reach ambient temperature and tap unopened on a solid surface to ensure that most of the lyophilized material is at the bottom of the vial. Gently remove the cap, add the desired volume of reconstitution medium, re-cap and mix thoroughly to bring all the oligosaccharide into solution. For maximal recovery of oligosaccharide, ensure that the cap lining is also rinsed and centrifuge the reconstituted vial briefly before use. Ensure that any glass, plasticware or solvents used are free of glycosidases and environmental carbohydrates. Minimise exposure to elevated temperatures or extremes of pH. High temperatures and low pH will cause desialylation. High pH will cause epimerisation of the reducing terminus GlcNAc.