A2F 2-AA Glycan Synonyms: AF2 N-linked oligosaccharide, FA2G2S2, FG2S2

Description: Di-sialylated, core-fucosylated bi-antennary complex-type N-glycan (oligosaccharide).

Sources: A2F glycan is found on many mammalian glycoproteins including thyroglobulin, gamma globulins, and IgG. This product is typically purified from the oligosaccharide pool released from porcine thyroglobulin by hydrazinolysis using a combination of HPLC and glycosidase digestion.

Form: Dry. Dried by centrifugal evaporation from an aqueous solution. Contains ammonium salt to stabilise against desialylation.

Molecular Weight: 2491

Number of Samples: Typically, 100 fmol to 10 pmol of the A2F 2-AA glycan can be detected, dependent on the sensitivity of the fluorometer, HPLC or mass spectrometer.

Amount of Sample :100 pmol

Structural Integrity: Determined by MALDI mass spectrometry and HPLC.

Labeling Selectivity :Essentially stoichiometric labelling. One fluorophore molecule covalently bonded to each glycan.

Detection: Fluorescence detection: 360 nm excitation wavelength, 425 nm emission wavelength.

Purity :>90% pure as assessed by a combination of 1 H-NMR and HPLC.

Storage: -20˚C both before and after dissolution. This product is stable for at least 5 years as supplied.

Shipping: The product can be shipped at ambient when dry.

Handling: Allow the unopened vial to reach ambient temperature and tap unopened on a solid surface to ensure that most of the lyophilized material is at the bottom of the vial. Gently remove the cap, add the desired volume of reconstitution medium, re-cap and mix thoroughly to bring all the oligosaccharide into solution. For maximal recovery of oligosaccharide, ensure that the cap lining is also rinsed and centrifuge the reconstituted vial briefly before use. Ensure that any glass, plasticware or solvents used are free of glycosidases and environmental carbohydrates. Minimise exposure to elevated temperatures or extremes of pH. High temperatures and low pH will cause desialylation. High pH will cause epimerisation of the reducing terminus GlcNAc.

Safety: This product is non-hazardous and has been purified from natural sources certified to be free of all hazardous material including pathogenic biological agents.