For in-depth pathway- or disease-specific gene expression analysis using LNA-enhanced, SYBR® Green-based PCR

Features

Build your own panel with any combination of assays from our cataloged Focus Panel assays

Easy online ordering through the intuitive custom builder tool

Exceptional sensitivity and specificity using short, LNA-enhanced primers

Stability for room-temperature setup allowing automation

Robust and reproducible results in less than 2 hours using the QuantiNova SYBR® Green PCR Kit

Product Details

QuantiNova LNA PCR Flexible Panels enable quick and reliable gene expression profiling using SYBR® Green-based qPCR. Simply tailor one of our existing Focus Panels to your requirements, or build a new panel containing assays from our cataloged Focus Panels. LNA enhancement enables us to design shorter primers with optimal Tm, allowing flexible positioning on the target for optimal sequence discrimination. Thorough design validation ensures top performance and robust detection. Optimized using QuantiNova chemistry, the simple workflow can be completed in just 2 hours and is robust enough to support room-temperature setup, automation and delayed run. We also offer Custom Panels, which are fully customizable with the predesigned and/or custom assays of your choice.

Performance

Expert LNA enhancement for top qPCR performance

The assays in the QuantiNova LNA PCR Flexible Panels have been developed using stringent design criteria and lab-validated algorithms. Twenty years of LNA design experience has enabled us to optimize our sophisticated LNA design algorithm, which incorporates over 50 different parameters to guarantee the most optimal assay for successful target detection. Each primer set delivers the highest specificity and efficiency for the most reliable and accurate results. LNA enhancement enable Tm normalization across the panel, giving the primers higher binding affinity, dramatically increasing assay sensitivity and specificity.

The increased sensitivity ensures excellent amplification efficiencies down to 1 RNA molecule, making it easier for you to detect low-abundance targets such as lncRNAs from less starting material . Increased specificity from the clever placement of LNA gives you a high signal-to-noise ratio, allowing discrimination of sequences that differ by only a single nucleotide and eliminating non-specific amplification and primer-dimer formation.

The high binding affinity of LNA bases increases the flexibility of primer placement on the transcript, so we are able to use intelligent positioning to design assays for otherwise difficult-to-analyze targets. This gives you robust and reliable quantification, even for AU-rich targets, low-abundance transcripts, targets with high secondary structure and highly complex samples.

Principle

High-performance SYBR® Green-based detection

QuantiNova LNA PCR Flexible Panels use SYBR® Green-based detection, which, when used with a highly specific PCR system, well-designed primers and optimized reaction conditions, offers several advantages over hydrolysis probe detection. First, it is possible to perform melting curve analysis immediately following the PCR, so you can confirm high specificity of the reaction and rule out formation of primer–dimers or non-specific amplicons. It also provides higher flexibility in the assay design, because you only need two primers, instead of two primers and a probe. Furthermore, while the primers ensure the specificity of the detection, the dye’s binding properties enable analysis of many different targets without having to synthesize target-specific, labeled probes, reducing your assay setup and running costs.

Choose from assays on our cataloged Focus Panels

QuantiNova LNA PCR Focus Panels include the assays needed for a thoroughly researched panel of relevant pathway- or disease-focused genes. The predesigned assays have been designed using our proprietary algorithm to provide the most sensitive, accurate and effective analysis. Most of the assays are intron-spanning when possible and detect only RNA. Assays that do not span an intron are designated as such, and if there is one exon in the target, unwanted signals can be easily eliminated using the QuantiNova Reverse Transcription Kit with the integrated gDNA removal step.

Flexible Panel vs. Custom Panel

QuantiNova LNA PCR Flexible Panels are a semi-customizable product and are available in 96-well plate, 384-well plate and 100-well disc formats, with up to 96 different assays on each plate. Flexible Panels can only contain assays from our available Focus Panels. Our convenient custom builder allows you to simply load the plate content of an existing Focus Panel and swap out the assays to meet your requirements. Reference gene assays and controls from our Focus Panels can also be added.

QuantiNova LNA PCR Custom Panels are a fully customizable product and are also available in 96-well plate, 384-well plate and 100-well disc formats, with all of our different plate layouts available. The custom builder allows you to upload your own list of targets and add any predesigned or custom assays of your choice. Any reference genes and control assays and controls can also be added.

Plate quantity ordered Discount (%)

1–5                                        0

6–11                               15

12–23                               30

24+                                       50

Complimentary data analysis

The complimentary, web-based data analysis tool in the GeneGlobe Data Analysis Center includes a user-friendly wizard to guide you step-by-step through the normalization and analysis of your data and generates publication-ready results figures.

Procedure

Choose one- or two-step qRT-PCR

QuantiNova LNA PCR Flexible Panels have been optimized using the time-tested and high-performance QuantiNova chemistry. Best results are obtained when reverse transcription is performed with the QuantiNova Reverse Transcription Kit. The resulting cDNA is then quantified by qPCR using the master mixes of the QuantiNova SYBR® Green PCR Kit combined with the assays on your panel.

For one-step qRT-PCR, we recommend using the QuantiNova SYBR® Green RT-PCR Kit, which offers quick reaction setup without need for optimization of reaction conditions. Simply combine RNA template with the ready-to-use RT-PCR master mix and the assays on your panel and start the reaction.

Fast and easy workflow

The fast and easy workflow can be completed in just 2 hours with minimal hands-on time and can be automated to save even more time and labor. The simple protocol has been tested for compatibility on all major brand qPCR cyclers . Furthermore, the cDNA generated in the RT step can be used across the entire QuantiNova LNA PCR System, allowing seamless transition from assays to panels depending on your research needs, saving you time and sample.

What you need to get started with the QuantiNova LNA PCR system

Reverse transcription: QuantiNova Reverse Transcription Kit

qPCR mastermix: QuantiNova SYBR® Green PCR Kit

One-Step qRT-PCR (optional): QuantiNova SYBR® Green RT-PCR Kit

Assays or panels:

QuantiNova LNA PCR Assays

QuantiNova LNA PCR Reference Assays

QuantiNova LNA PCR Custom Assays

QuantiNova LNA PCR Focus Panels

QuantiNova LNA PCR lncRNA Focus Panels

QuantiNova LNA PCR Custom Panels

QuantiNova LNA PCR Flexible Panels

Applications

QuantiNova LNA PCR Flexible Panels are highly suited for applications including:

mRNA and lncRNA expression analysis, profiling and quantification

Validation of RNA-seq gene expression data

Gene expression profiling

Signal and pathway analysis

Confirming gene expression knockdown by LNA GapmeRs or siRNAs

Biomarker development, including screening, identification and validation of disease-associated biomarkers

Monitoring phenotypic changes related to gene expression