Murine Anti-Factor IX
Factor IX (FIX) is a vitamin K-dependent zymogen that plays an essential role in the coagulation cascade leading to thrombus formation. In the presence of calcium, activated Factor IX (FIXa) complexes with Factor VIIIa on phospholipid surfaces to create the tenase complex, which converts Factor X to its activated form. Absent or defective FIX is the cause of the X-linked recessive bleeding disorder hemophilia B. GMA-124 binds to FIX and detects the light chain of FIXa in both ELISA and Western blot formats. In addition, bound GMA-124 captures FIX by bio-layer interferometry.
物种反应性: Bovine, Human, Porcine, Rat
特异性: Light chain of FIX/FIXa
免疫原: Human Factor IX
ELISA: Binds immobilized Human, Rat, Porcine, and Bovine FIX, Human FIXa
Immunoblotting: Western blot detects Human FIX, light chain of Human FIXa under reduced conditions.
Inhibition: Does not prolong plasma clot time in APTT clotting assay.
BLI: KD=50 nM (kdis=4.38x10-3 sec-1) by bio-layer interferometry.