The addition of an arginine to glutamic acid mutation generates human tPA that is 100percent single chain and resistant to cleavage by plasmin. The mutation site is R275E on the mature protein and R310E on the complete mRNA sequence (UniProtKB: locus TPA_HUMAN, accession P00750). Recombinantly produced in insect cells.
References:
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KM Tate, DL Higgins, WE Holmes, ME Winkler, HL Heyneker, and GA Vehar
Biochemistry, January 27, 1987; 26(2): 338-43.
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HR Lijnen, B Van Hoef, L Nelles, and D Collen
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