ATA-Phe-Pro-Arg-CMK
货号:INFPRCK
|
规格:1.0 mg
|
价格:¥0.00
|
品牌:Molecular Innovations
ATA-Phe-Pro-Arg-CMK是由北京中昊新生科技有限公司提供,Molecular Innovations专注于凝血和溶栓试剂,包括PAI-1116、tPA、尿激酶、凝血因子、玻连蛋白、纤维蛋白原、纤连蛋白、肾素原/肾素、前激肽释放酶、激肽释放酶、白蛋白、纤溶酶原、纤溶酶、抗纤溶酶、凝血酶、抗凝血酶、补体成分和免疫球蛋白。
ATA-FFRCK (Na-[(acetylthio) acetyl]-D-Phe-Phe-Arg-CH2Cl) and ATA-FPRCK (Na-[(acetylthio)acetyl]-D-Phe-Pro-Arg-CHCl2) are active-site specific labeling reagents for serine proteases. They are derivatized from irreversible peptide chloromethyl ketones and facilitate incorporation of a thioester moiety via alkylation of the catalytic site Histidine residue. Liberation of the free thiol group is accomplished by gentle treatment with hydroxylamine (NH2OH) following irreversible incorporation into the enzyme catalytic site. The free thiol then becomes a site for specific modifications with thiol-reactive probes such as iodoacetamide fluorescent probes. Many serine proteases in which free thiols are lacking may be specifically labeled at the active site by these reagents. Both ATA-FPRCK and ATA-FFRCK have been used to label thrombin with 5-(iodoacetamido) fluorescein (5-IAF). The probe was then effectively utilized to follow conformational changes in the catalytic domain of alpha-thrombin upon binding to the fragment 2 domain of prothrombin. In addition, quantitative equilibrium binding studies and investigations into the kinetics underlying the non-proteolytic activation of the zymogen plasminogen by streptokinase were characterized with 2-((4'-iodoacetamido) anilino) naphthalene-6-sulfonic acid (IAANS) labeled plasminogen by using the ATA-FFRCK reagent. References: 1. Bock, P. (1993) Method Enzymol. 222:478-503. 2. Bock, P. et al. (1996) J Biol. Chem. 271:1072-1080.
相关产品
-
货号:HFIII
|
规格:0.025 mg
|
价格:¥0.00
|
品牌:Molecular Innovations
-
货号:SASHCRP-GF-FITC
|
规格:1.0 mg
|
价格:¥0.00
|
品牌:Molecular Innovations
-
货号:ASMATIII-GF-FITC
|
规格:1.0 mg
|
价格:¥0.00
|
品牌:Molecular Innovations
-
货号:SASMTPA-GF-FITC
|
规格:1.0 mg
|
价格:¥0.00
|
品牌:Molecular Innovations
-
货号:SASHCRP-GF-FITC
|
规格:10 mg
|
价格:¥0.00
|
品牌:Molecular Innovations
-
货号:HHCII
|
规格:1.0 mg
|
价格:¥0.00
|
品牌:Molecular Innovations
-
货号:MUPA-AF700
|
规格:0.1 mg
|
价格:¥0.00
|
品牌:Molecular Innovations
-
货号:HFVIIKT-TOT
|
规格:1 kit
|
价格:¥0.00
|
品牌:Molecular Innovations