TaqRobat Native Taq DNA Polymerase is a recombinant Taq that has identical characteristics to native Taq from Thermus aquaticus. This highly robust enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa, and will create products with dA overhangs at 3' ends. Suitable for Standard Endpoint PCR, Colony PCR, Genotyping, and TA Cloning of DNA templates up to 5kb. TaqRobat offers higher yields than some leading competitors with excellent lot-to-lot reproducibility. TaqRobat undergoes extensive quality control and is free of contaminating endonucleases.
Supplied with your choice of complimentary MgCl2 or MgCl2 free 10X PCR reaction buffer.
Thermal cycling conditions: The following general cycling conditions are recommended but can vary depending on the template and primers being used.
| Cycling Step | Temperature | Holding Time | Cycles |
|---|
| Initial Denaturation | 94°C | 2min | 1 |
| Denaturation | 94-96°C | 30sec-4min | 20-30 |
| Annealing* | 55-65°C | 15-30sec |
| Extension | 70-72°C | 30sec-1min per kb |
| Final Extension | 70-72°C | 0-10min | 1 |
*Annealing temperature will depend on primer length and composition. Generally, begin 5°C below primer Tm.
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