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Human PAI-1 (N-terminal cysteine substrate form stable mutant)
货号：NTCYSCPAI-T333R | 规格：0.5 mg | 价格：￥0.00 | 品牌：Molecular Innovations
Human PAI-1 (N-terminal cysteine substrate form stable mutant)是由北京中昊新生科技有限公司提供，Molecular Innovations专注于凝血和溶栓试剂，包括PAI-1481、tPA、尿激酶、凝血因子、玻连蛋白、纤维蛋白原、纤连蛋白、肾素原/肾素、前激肽释放酶、激肽释放酶、白蛋白、纤溶酶原、纤溶酶、抗纤溶酶、凝血酶、抗凝血酶、补体成分和免疫球蛋白。
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This stable form of PAI-1 contains an N-terminal cysteine mutation in addition to the four stabilization point mutations. A single substitution at position P14 in the reactive center loop produces a PAI-1 that becomes a substrate for proteinases rather than an inhibitor. The protein is supplied in buffer with 1mM DTT to prevent dimerization. To label with thiol reactive dye, buffer exchange into degassed 0.05M Sodium Phosphate; 0.1M NaCl; 1mM EDTA; pH 6.6 by desalting column. Collect into 10 fold molar excess of maleimide or iodoacetamide dye and incubate for 1 hour at room temperature. To preserve PAI-1 activity minimize the percentage of DMSO/DMF in the reaction. Remove free dye by desalting, ammonium sulfate precipitation, or dialysis.
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