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Human PAI-1 (N-terminal cysteine, active fraction)
货号：NTCYSPAI-A | 规格：0.5 mg | 价格：￥0.00 | 品牌：Molecular Innovations
Human PAI-1 (N-terminal cysteine, active fraction)是由北京中昊新生科技有限公司提供，Molecular Innovations专注于凝血和溶栓试剂，包括PAI-1483、tPA、尿激酶、凝血因子、玻连蛋白、纤维蛋白原、纤连蛋白、肾素原/肾素、前激肽释放酶、激肽释放酶、白蛋白、纤溶酶原、纤溶酶、抗纤溶酶、凝血酶、抗凝血酶、补体成分和免疫球蛋白。
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Wild type PAI-1 cloned with a cysteine residue at the N-terminus (patent pending). The active fraction is >95percent pure and >95percent active as determined by titration with HWM tc-urokinase and SDS PAGE. The protein is supplied in buffer with 1 mM DTT to prevent dimerization. To label with thiol reactive dye, buffer exchange into degassed 0.05M Sodium Phosphate; 0.1M NaCl; 1mM EDTA; pH 6.6 by desalting column. Collect into 10 fold molar excess of maleimide or iodoacetamide dye and incubate for 1 hour at room temperature. To preserve PAI-1 activity minimize the percentage of DMSO/DMF in the reaction. Remove free dye by desalting, ammonium sulfate precipitation, or dialysis.
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