Rat Heart Tissue Lysate
货号:RT-TL-HT
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规格:0.2 mg
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价格:¥0.00
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品牌:Molecular Innovations
Rat Heart Tissue Lysate是由北京中昊新生科技有限公司提供,Molecular Innovations专注于凝血和溶栓试剂,包括PAI-1795、tPA、尿激酶、凝血因子、玻连蛋白、纤维蛋白原、纤连蛋白、肾素原/肾素、前激肽释放酶、激肽释放酶、白蛋白、纤溶酶原、纤溶酶、抗纤溶酶、凝血酶、抗凝血酶、补体成分和免疫球蛋白。
A freshly-harvested Sprague Dawley rat heart is flash-frozen in liquid nitrogen within 30 seconds of removal and stored frozen until lysis. The tissue is ground and thoroughly homogenized at 4C with a freshly prepared modified Radio Immuno Precipitation Assay (RIPA) buffer containing strong protease and phosphatase inhibitor cocktails. The final homogenate is incubated with DNase then clarified by centrifugation at 10,000 g at 4C and stored at -80C. Each lot is quality controlled by determining the protein concentration using a BCA assay and analyzed with SDS-PAGE using Coomassie blue for visualization. Sharp, clear protein bands indicate a quality protein lysate.
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