钙结合蛋白-D28抗体

The antibody has a proven maximum biotin-streptavidin/HRP staining at a 1/5,000 – 1/10,000 dilution in rat striatum, cortex, and hippocampus.

The antiserum has been characterized as specific to calbindin D-28k; please see reference listed below. Optimal dilution will vary depending upon fixation, labeling technique and/or detection system; therefore, a dilution series is recommended.

照片描述: IHC image of neurons staining for the calbindin in the rat amygdala (above) and the rat cortex (below). The tissue was fixed with 4% formaldehyde in 0.1 M phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:10000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen. The section was then mounted on slides with permount.

宿主: Rabbit

规格/体积: 100 µL

性状: Lyophilized Whole Serum

反应性: Mouse, Rat

别名:  Calbindin; nCAB27; Calbindin D28; D-28K; Vitamin D-dependent calcium-binding protein; Vitamin D-dependent calcium-binding protein, avian-type; CALB1; calbindin 1, 28kDa, anti-Calbindin

RRID:  AB_572222

免疫原: Bovine Calbindin D-28K

Gene Symbol: CALB1