神经降压素抗体

The ImmunoStar Neurotensin antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat amygdala using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilution is 1/4000-1/8000 in PBS/0.3% Triton x-100 – Bn/Av-HRP Technique. Staining is completely eliminated by pretreatment with 10 µg of Neurotensin per 1 mL of diluted antibody.

照片描述: IHC image of rat amygdala staining for neurotensin. The tissue was fixed with 4% formaldehyde in 0.1 M phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:5000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen.

宿主: Rabbit

规格/体积:  100 µL

性状: Lyophilized Whole Serum

反应性: Cat, Dog, Frog, Human, Monkey, Mouse, Pigeon, Rat, Turtle

别名:  neuromedin N; Preproneurotensin; pro-neurotensin, anti-neurotensin

Gene Symbol:  NTS