大鼠GIP抗体

Anti-GIP (Active) (Rat) Monoclonal Antibody

Description: This antibody,which specifically recognized to rat GIP(1-42)active form was raised in a mouse by immunization with  a keyhole limpet hemocyanin(KLH)conjugate of synthetic rat GIP.The product vial contains 0.05 mg purified IgG₁of the titled antibody obtained by lyophilizing its 0.01M PBS(pH 7.2,0.1 mL) solution without preservative.It can be used for immunoassay or any other immunoreaction with GIP(1-42) activeform.

Immunogen: Synthetic rat GIP-Cys -KLH conjugate                           

Host: Mouse

Amino Acid Sequence GIP(rat):

YAEGTFISDY      SIAMDKIRQQ      DFVNWLLAQK      GKKNDWKHNL      TQ

Isotype: IgG(Protein  A  purified)

Product Form: Lyophilized purified IgG

Reconstitution: Reconstitute the product with 0.1 mL distilled water to make 0.5 mg/mL stock solution of 0.01M PBS.If it is stored in a refrigerator,add moderate antiseptic to the solution (e.g.NaN₃0.1%)

Storage: The product will be stable for over one year if it be stored at -20℃ to -80℃ until opened.Upon reconstitution,the  antibody  solution  must  be  stored  at  2℃  to  8℃  and  used  within  one  month.Repeated freezing-thawing should be avoided.

Suggested Working Dilution Range: 1:100 for immunohistochemistry (frozen or paraffin section).Optimal dilution should be determined by each laboratory for each application

Positive Control: (immunohistochemistry):Rat duodenum

Species Tested: Rat

Specificity(based on EIA): Rat GIP(1-42)(Active form) 100%,Mouse GIP(1-42) (Active form) 100%, Human GIP(1-42)(Active form)100%,RatGIP(3-42)(Inactive          form)0%,Mouse GIP(3-42)(Inactive form) 0%,Human GIP(3-42)(Inactive form)0%,Glucagon 0%,GLP-1(7-36)NH₂0%,GLP-1(9-36)NH₂0%,Rat GLP-20%,Mouse GLP-20%,Human GLP-20%