PDPH crosslinker (3-[2-Pyridyldithio]propionyl hydrazide) is a cleavable, carbohydrate reactive, heterobifunctional protein crosslinking reagent. The PDPH crosslinker's hydrazide group selectively reacts with carbohydrates oxidized by sodium meta-periodate (or carboxyls when used with EDC); while the pyridinyldisulfide moity is reactive toward reduced thiols (sulfhydryls; -SH). Thus, PDPH protein crosslinker is an excellent choice for crosslinking glycoproteins, glycans, and other polysaccharides.

Additionally, the pyridyldisulfide group of the PDPH crosslinking reagent contains a cleavable disulfide (-S-S-) bond which can be reduced with TCEP-HCl or DTT to expose a thiol moity that can be further reacted with, for example, a maleimide containing crosslinker. Thus, PDPH may also be used as a thiolation reagent to add sulfhydryl functional groups to carbohydrate containing molecules.

PDPH溶于pH为5.5 0.1 M的醋酸钠中，浓度为14.2 mg/mL。

分子量: 229.32

间隔长度: 9.2 Å

别名: SPDP Hydrazide; PDPH Cross-linker; PDPH Cross Linker; S-(2-thiopyridyl)-3-mercaptopropionic acid hydrazide; TPMPH; 3-(2-pyridyldithio)propionyl hydrazide