NHS-SS-Biotin
货号:b2105-100mg
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规格:100 mg
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价格:¥0.00
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品牌:ProteoChem
NHS-SS-Biotin 是由北京中昊新生科技有限公司提供.ProteoChem致力于蛋白生物化学和有机合成领域的生物试剂和化学试剂研发,生产NMR、质谱、蛋白偶联剂以及新型蛋白质组学试剂的分析标准品,用于质谱,药物研发,诊断和蛋白生物中的蛋白检测和蛋白修饰等方面。
NHS-SS-Biotin, Succinimidyl-2-(biotinamido)-ethyl-1,3'-dithiopropionate, is an amine-reactive biotinylation reagent that is cleavable when the disulfide (-S-S-) bond is reduced. NHS-SS-Biotin is cell membrane permeable and has a long chain separating the biotin group from the amino-reactive NHS-ester (N-Hydroxysuccinimide ester). The resulting biotinylated protein can then be detected when used in conjunction with streptavidin.
It is possible to cleave the biotin group from the crosslinked target protein under reducing conditions, such as in the presence of beta-mercaptoethanol (BME), TCEP-HCl or DTT. This technique is often used to purify an antibody or other protein from a biotin specific affinity resin like Immobilized Streptavidin without exposing your protein of interest to denaturing conditions.
Molecular Weight: 504.65
Spacer Length: 24.3 Å
CAS Number: 142439-92-7
Alternate Names: Biotin disulfide N-Hydroxysuccinimide ester; NHS SS Biotin
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