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Immobilized Pepsin
货号：g4106-50ml | 规格：50 ml | 价格：￥0.00 | 品牌：ProteoChem
Immobilized Pepsin 是由北京中昊新生科技有限公司提供.ProteoChem致力于蛋白生物化学和有机合成领域的生物试剂和化学试剂研发，生产NMR、质谱、蛋白偶联剂以及新型蛋白质组学试剂的分析标准品，用于质谱，药物研发，诊断和蛋白生物中的蛋白检测和蛋白修饰等方面。
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Immobilized Pepsin resin (Pepsin Agarose) is commonly used to create F(ab’)2 fragments from antibodies. By immobilizing pepsin autolysis and protease contamination is essentially eliminated. Immobilized Pepsin gel hydrolyzes protein non-specifically at pH 1.0 to 3.0, with an optimum activity at pH 1.0. ProteoChem's highly active pepsin is covalently bound to crosslinked 6% beaded agarose resin and its specific activity is > 6,100 units/ml resin (one unit is defined as releasing 0.001 A280 as TCA hydrolyzed/min. at 37°C). ProteoChem's Immobilized Pepsin is packaged as a slurry in buffer at pH 4.5 containing 50% glycerol, 100 mM Sodium Acetate, 0.05% NaN3.

Pepsin protease cleaves non-specifically with some preference for tyrosines and phenylalanine (aromatic residues), although it generally does not cleave at alanine, glycine or valine residues. Recommended conditions are pH 1.0-4.5, 20°C to 37°C.
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