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Protocol ：
Actual free detergent concentration in biological samples can vary greatly,
so the ratios shown are only intended to provide general guidance in use,
refer to chart above.
1. Resuspend SurfactAwayTM by gently shaking. Excessive shaking or mixing
will cause foaming.It should be completely resuspended prior to use.
2. Add 1 ml of SurfactAwayTM to 4 ml of the sample. (1 : 4 ratio). Mix the
sample by gently shaking periodically for 10 minutes.
3. Centrifuge sample at 16,000 G’s for 1 minute - or - 1,000 G’s for 15 minutes.
4. Decant supernatant containing macromolecules of interest and continue
with purification.
5. Different sample volumes are easily scaled. Volume ratio can be adjusted
up or down as required to remove the desired amount of detergent.
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