For simultaneous purification of viral DNA and RNA from plasma, serum and cell-free body fluids. For 50 minipreps: 50 QIAamp MinElute Columns, QIAGEN Protease, carrier RNA, buffers, Collection Tubes (2 ml).

Features

Maximal concentration in eluate for high downstream sensitivity

Rapid purification of high-quality viral DNA and RNA

No organic extraction or alcohol precipitation

Consistent, high yields

Removal of contaminants and inhibitors

Product Details

QIAamp MinElute Virus Kits simplify viral DNA and RNA purification with fast spin-column and vacuum procedures. The kits use starting sample volumes of up to 0.2 ml and combine the selective binding properties of a silica-based membrane with flexible elution volumes of between 20 and 150 μl. The QIAamp MinElute Virus Spin process can be fully automated on the QIAcube Connect. The additional buffers and reagents in the QIAamp MinElute Virus Accessory are required for automation of the QIAamp MinElute Virus Spin procedure.

Performance

Purified viral nucleic acids are free of proteins, nucleases and other impurities, and are suitable for use in sensitive downstream applications such as PCR and RT-PCR .

Viral nucleic acids purified using QIAamp MinElute Virus Kits can be used in a wide range of downstream applications, including:

PCR and quantitative real-time PCR

Infectious disease research

Principle

QIAamp MinElute Virus Kits simplify the isolation of viral RNA and DNA from plasma, serum and cell-free body fluids with a fast spin-column or vacuum procedure. No phenol–chloroform extraction is required. Nucleic acids bind specifically to the QIAamp MinElute silica-gel membrane while contaminants pass through. PCR inhibitors, such as divalent cations and proteins, are completely removed in two efficient wash steps, leaving pure nucleic acids to be eluted in either water or a buffer provided with the kit. QIAamp MinElute technology yields viral DNA and RNA from serum, plasma and cell-free body fluids that are ready to use in PCR and blotting procedures.

QIAamp MinElute Virus Kits combine the selective binding properties of a silica-based membrane with flexible elution volumes of between 20 and 150 µl. The QIAamp MinElute Virus Spin procedure can be fully automated on the QIAcube Connect. The additional buffers and reagents provided in the QIAamp MinElute Virus Accessory Set are required.

QIAamp sample preparation technology is fully licensed.

Procedure

Optimized buffers lyse samples, stabilize nucleic acids and enhance selective DNA adsorption to the QIAamp MinElute membrane  Alcohol is added and lysates loaded onto the QIAamp MinElute spin column. Wash buffers are used to remove impurities and viral nucleic acids are eluted in Buffer AVE, ready for use in amplification reactions or storage at –20ºC. Purified nucleic acids are free of proteins, nucleases and other impurities.

Applications

The QIAamp MinElute Virus Spin Kit uses well-established technology for simultaneous purification of viral RNA and DNA from fresh or frozen plasma, serum, other cell-free body fluids.

The QIAamp MinElute Virus Vacuum Kit uses well-established technology for simultaneous purification of viral RNA and DNA. Fresh or frozen plasma, serum and other cell-free body fluids (500 µl) can be processed in less than an hour.