顺势表达N端标记6xHis的标签蛋白

Features

严谨调控的蛋白表达，增强蛋白结构的细胞内稳定性

不需要第二个质粒的反式抑制子

氨苄青霉素或卡那霉素抗性

卡那霉素抗性确保可靠的克隆，以及更稳定的蛋白表达

The vectors in the cis-Repressed pQE Vector Set are similar to those in the N-Terminus pQE Vector Set, but additionally express the laclq gene product that represses protein expression prior to IPTG induction. This eliminates the need to include this gene on another plasmid. Use of these vectors is recommended when expressing gene products toxic to E. coli.

Performance
The cis-Repressed pQE Kan Vector Set contains vectors with a kanamycin resistance gene in addition to the ampicillin resistance gene providing selection by either antibiotic.

Principle
pQE vectors provide high-level expression of protein in E. coli and add the small 6xHis affinity tag to protein expression constructs.

Procedure
Once a protein-encoding DNA insert has been incorporated into the vector, it is transformed into E. coli cells and protein expression is induced by the addition of IPTG to the growth medium. After cell lysis, 6xHis-tagged proteins are purified by Ni-NTA affinity chromatography under native or denaturing conditions.

Applications
The pQE vectors in this product provide high-level expression of protein in E. coli , enabling:
Purification of functional proteins under native conditions
Purification under denaturing conditions for antibody generation
Efficient detection using Anti·His antibodies and conjugates