使用FPLC纯化6xHis-tagged蛋白
Features
从细胞粗提液中纯化蛋白质只需一步,纯度高于95%
高亲和力和高结合度
可选择在天然或变性条件下纯化蛋白
预装的即用型基质适合多种规模的蛋白纯化
提供自动纯化和分析的方法
Supporting data and figures
Ni-NTA Superflow Cartridges
For His-tagged protein purification using liquid chromatography systems
Specifications
Features Specifications
Applications Proteomics
Scale Large scale
Processing Manual
Bead size 60–160 µm
FPLC Yes
Special feature Batch and column purification
Gravity flow or spin column Gravity flow or automated
Binding capacity Up to 50 mg/ml
Start material Cell lysate
Support/matrix Superflow
Tag 6xHis tag
Yield Depends on binding capacity
Number of preps per run 1–24 samples per run