For rapid removal of unwanted RNAs during RNA-seq library preparation

Features

Specify up to 20,000 bases of RNAs for removal

Stable for at least 18 months when stored as directed

Only one pipetting step – combine QIAseq FastSelect reagent with RNA and incubate

Works for QIAGEN, Illumina, NEB, KAPA and other stranded RNA-seq library kits

Performs equally well with high-quality or highly fragmented RNA

Product Details

QIAseq FastSelect Custom RNA Removal Kits use a novel method to remove highly abundant RNA that is of low scientific value from your RNA-seq libraries. You can now design your own custom QIAseq FastSelect pools to remove any RNAs you wish from your RNA-seq library. Simply paste the RNA sequences you want removed into the QIAseq FastSelect Custom Builder to generate a unique catalog number for your custom FastSelect pool.

Principle

Removing highly expressed, but biologically unimportant, RNA transcripts makes NGS more efficient and enables higher sample throughput with higher sensitivity. Furthermore, removal of unwanted RNA species from FFPE (formalin-fixed paraffin-embedded) samples and from degraded RNA samples is particularly challenging and can result in suboptimal performance.

QIAseq FastSelect is designed for quick, efficient removal of unwanted RNAs from total RNA during NGS RNA-seq library preparation. QIAseq FastSelect seamlessly integrates with your existing RNA stranded library preparation workflow for RNA removal in a single, 14-minute inline step. Prior to RNA heat fragmentation (which is optional and dependent upon the library preparation kit and sample type), QIAseq FastSelect removal reagent is directly combined with total RNA and the library preparation-specific buffers. After fragmentation, the reaction temperature is stepwise cooled to room temperature and the remaining library preparation steps are completed. There is no need to perform any type of enrichment on the total RNA samples. QIAseq FastSelect Kits ensure consistently high performance with RNA amounts ranging from as little as 1 ng to 1 µg. QIAseq FastSelect can be used with RNA from fresh samples, as well as RNA from FFPE samples or degraded RNA, and delivers reliable rRNA removal and high reproducibility in downstream applications.

Procedure

Most RNA removal or depletion strategies associated with RNA-seq library construction are sample pre-treatment strategies involving hybrid-capture or enzymatic removal of unwanted RNA. Our unique QIAseq FastSelect procedure is compatible with QIAGEN, Illumina, KAPA and NEB stranded library preparation kits and provides complete rRNA removal in a single, 14-minute inline step (see figure " QIAseq FastSelect Kit workflow"). This is dramatically faster than alternative RNA depletion kits, which require pre-treatment protocols involving more than 25 steps and 2 hours to complete.

Simply add QIAseq FastSelect reagent to the RNA sample, perform fragmentation (if required), stepwise cool the reaction from 75°C to 25°C for 14 minutes and then complete the remaining library preparation steps. QIAseq FastSelect works with or without RNA fragmentation, providing the flexibility to use RNA from FFPE samples or degraded RNA samples, or high-quality RNA as part of a standard or FFPE RNA-seq library construction workflow.

Applications

QIAseq FastSelect delivers rapid, reliable RNA removal from FFPE and fresh sample RNA sources. QIAseq FastSelect Kits are available in a variety of different formats and sizes to suit your specific applications, such as RNA extraction troubleshooting.