For unprecedented ease-of-use and biological insights using targeted DNA sequencing

Features

Less than 6 hours total turnaround time to go from setup to sequencing

Flexibility to multiplex QIAseq Targeted DNA Pro Custom libraries with other chemistries

New enzymatic process for preventing adapter and primer carryover contamination, eliminating time-consuming bead purifications

Reveal structural variants with breakpoints defined at the nucleotide level that span multiple exons, in addition to SNPs and indels

Product Details

QIAseq Targeted DNA Pro Custom Panels set a new standard for NGS genotyping. Major innovations within this new chemistry provide improved ease-of-use, efficiency and expanded variant analysis. This highly optimized DNA target enrichment technology offers an automation-friendly solution that simultaneously facilitates ultrasensitive variant detection using integrated unique molecular indices (UMIs). It is a powerful tool for the detection of genetic variations including somatic mutations, single nucleotide polymorphisms, copy number variation and small insertions/deletions. In addition to our catalog panels, fully customized designs are easily created. Kits are optimized for use with our QIAseq Targeted Pro Panel Unique Dual-Index primers for use with Illumina sequencing systems, as well as Ion-compatible adapters for sequencing on Ion platforms.

Performance

Compatible with diverse sample types including cells, tissue and biofluids, catalog panels target the entire coding region of relevant oncogenes and tumor suppressor genes, as well as actionable and interpretable cancer-related somatic and germline variants.

Building on our expertise employing UMIs to correct for PCR and sequencing errors, coupled with our proprietary highly specific target enrichment, QIAseq Targeted DNA Pro Custom Panels offer a streamlined library preparation workflow that takes less than 6 hours with minimal hands-on time. The simplicity of QIAseq Targeted DNA Pro Custom Panels is suitable for routine known and novel cancer mutation detection in any research laboratory with access to Illumina and Ion Torrent NGS platforms

Principle

QIAseq Targeted DNA Pro Custom Panels are provided as single-tube primer mixes for a single sequencing reaction with the required amount of template ranging from 10–80 ng for fresh DNA or 100–250 ng for formalin-fixed paraffin-embedded (FFPE) DNA.

Due to the fragmented and modified nature of FFPE DNA samples, many NGS library construction workflows have a low recovery rate from FFPE DNA, whereas QIAseq Targeted DNA Pro Custom incorporates a seamless FFPE DNA repair step before library construction. The specially formulated chemistry achieves highly efficient enrichment even in GC-rich regions. In addition, QIAseq Targeted DNA Pro Custom libraries can be sequenced with Illumina default sequencing primers and are compatible with most mid- to high-throughput Illumina sequencers. Alternative targeted DNA library construction methods have multiple bead cleanup steps that are labor-intensive and result in inconsistent library yield. By replacing the bead cleanup steps with enzymatic processes after the ligation and target enrichment steps, QIAseq Targeted DNA Pro Custom enables a highly efficient and automation-friendly workflow. Data analysis tools have been optimized for seamless variant calling and additional analysis features are supported through QIAGEN Digital Insights solutions.

Procedure

The entire workflow for QIAseq Targeted DNA Pro Custom Panels goes from extracted DNA to sequencing-ready libraries within 6 hours. Extracted DNA is fragmented, genomic targets are molecularly barcoded and enriched, and libraries are constructed with an enzymatic cleanup after ligation and target enrichment. Data analysis is performed using the QIAseq Targeted DNA Pro Analysis software pipeline or QIAGEN CLC Genomics Workbench. Furthermore, detected variants can be annotated with QIAGEN Clinical Insight Interpret for QIAseq.

Applications

QIAseq Targeted DNA Pro Custom Panels can be used to call a variety of DNA variants from a wide range of sample types for many applications.

DNA variants:

SNVs

Small indels

CNVs

Select structural variants with well-characterized breakpoints

Loss of heterozygosity (LOH)

Sample types:

FFPE

Plasma/serum

Fresh or frozen tissue

Cell lines

Applications:

Profiling of DNA variants in solid tumor and hematologic malignancies

Hotspot detection in solid tumors

Examination of variants in mitochondrial DNA