For rapid rRNA removal for RNA-seq library preparation from plant samples

Features

Compatible with QIAGEN, Illumina, NEB and KAPA stranded RNA-seq library kits

Single reagent for plant leaves, stems and roots

High-performance cytoplasmic, mitochondrial and chloroplast plant rRNA removal in just 14 minutes

Only one pipetting step – combine QIAseq FastSelect reagent with RNA and incubate

No extra cleanup steps or NGS library protocol changes

Product Details

QIAseq FastSelect –rRNA Plant Kits use a novel method to remove highly abundant RNA that is of low scientific value from your RNA-seq libraries. Researchers using RNA-seq for whole transcriptome analysis can use QIAseq FastSelect –rRNA Plant Kits to remove cytoplasmic (5.8S, 18S and 25S), mitochondrial (5S, 18S and 26S) and chloroplast (4.5S, 5S, 16S and 23S) rRNA.

We recommend using QIAseq Stranded RNA Library Kits for robust strand-specific RNA-seq library preparation for both high-quality and highly fragmented RNA.

Design your own custom QIAseq FastSelect pools to remove any RNAs you wish from your RNA-seq library – take a look at our QIAseq FastSelect Custom RNA Removal Kits.

Want to try the QIAseq FastSelect –rRNA Plant Kit for the first time? Request a trial kit to evaluate.

Performance

QIAseq FastSelect –rRNA Plant Kits demonstrated highly efficient removal of rRNA and robust performance .

QIAseq FastSelect –rRNA Plant Kits also provide reproducible gene expression results  and consistent read mapping with a variety of plants.

Principle

Removing highly expressed, but biologically unimportant RNA transcripts makes NGS more efficient and enables higher sample throughput with higher sensitivity. Furthermore, removal of unwanted RNA species from full length and fragmented RNA samples can be particularly challenging, and can result in suboptimal performance.

QIAseq FastSelect –rRNA Plant Kits are designed for quick, efficient removal plant rRNA found in cytoplasmic, mitochondrial and chloroplast ribosomes from total RNA during NGS RNA library preparation. QIAseq FastSelect seamlessly integrates with your existing RNA stranded library preparation workflow for RNA removal in a single, 14-minute inline step. Prior to RNA heat fragmentation (which is optional and dependent upon the library preparation kit and sample type), QIAseq FastSelect removal reagent is directly combined with total RNA and the library preparation-specific buffers. After fragmentation, the reaction temperature is stepwise cooled to room temperature and the remaining library preparation steps are completed. There is no need to perform any type of enrichment on the total RNA samples. QIAseq FastSelect –rRNA Plant Kits ensure consistently high performance with RNA amounts ranging from as little as 1 ng to 1 μg. QIAseq FastSelect can be used with RNA from fresh samples from many species of plants, and delivers reliable rRNA removal and high reproducibility in downstream applications.

Procedure

Most RNA removal or depletion strategies associated with RNA-seq library construction are sample pre-treatment strategies involving hybrid-capture or enzymatic removal of unwanted RNA. Our unique QIAseq FastSelect procedure is compatible with QIAGEN, Illumina, KAPA and NEB stranded library preparation kits and provides complete rRNA removal in a single, 14-minute inline step . This is dramatically faster than alternative RNA depletion kits, which require pre-treatment protocols involving more than 25 steps and 2 hours to complete.

Simply add QIAseq FastSelect reagent to the RNA sample, perform fragmentation (if required), stepwise cool the reaction from 75°C to 25°C for 14 minutes and then complete the remaining library preparation steps. QIAseq FastSelect works with or without RNA fragmentation, providing the flexibility to use fragmented RNA from plant samples or high-quality RNA as part of a standard RNA-seq library construction workflow.

Applications

QIAseq FastSelect delivers rapid, reliable RNA removal from full length or fragmented RNA samples from many species of plants. QIAseq FastSelect –rRNA Plant Kits are available in a variety of different formats and sizes to suit your specific applications, such as whole transcriptome analysis.