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硝基酪氨酸ELISA试剂盒 Nitrotyrosine ELISA Kit
货号:NWK-NTR01-01 | 规格:96 well | 价格:¥0.00 | 品牌:Northwest Life Science
NWLSS™ 硝基酪氨酸ELISA试剂盒推荐用于检测血浆、血清和组织匀浆样品中的亚硝基化蛋白。特异性:亚硝基化蛋白质加合物;灵敏度:2 nM

NWLSS™硝基酪氨酸ELISA推荐用于检测血浆、血清和组织样本中的亚硝基化蛋白。硝基酪氨酸的检测是过氧硝酸盐相关研究的优秀工具,并推荐作为蛋白相关氧化/硝化应激相关增强的生物标记物。该检测是一种操作简单的“夹心法”ELISA。


Nitrate (NO3) and nitrite (NO2) production are typically used as markers of nitric oxide production (NO) production. These analytes by themselves fail to address the eventual fate of NO or the possible adverse effects associated with its excess production and reaction with free radical species in vivo. Active NO metabolites can react with superoxide to form peroxynitrite (ONOO-) a powerful oxidant and nitrating agent. Subsequent reaction of peroxynitrite with proteins results in nitrotyrosine formation. As a stable end product of peroxynitrite mediated oxidation/nitration, Nitrotyrosine can be used as a surrogate index of NO dependent damage in vivo and has been associated with multiple disease states.


Test Principle

The NWLSS™ Nitrotyrosine ELISA test is a simple "sandwich" ELISA using a plate bound capture antibody (anti nitrated KLH) to nitrotyrosine and a biotinylated secondary tracer antibody. Addition of streptavidin-peroxidase followed by tetramethylbenzidine (TMB) facilitates color development directly proportional to the nitrotyrosine present in the sample. The reaction is stopped using a citric acid solution and the assay is read on a plate reader at 450 nm.


格式:96孔可拆酶标板

预期用途:生物样品中硝基酪氨酸蛋白加合物的定量测量。 NWLSS™ 产品可以量化氧化/亚硝化应激对生物系统中蛋白质和过氧亚硝酸盐形成的影响。

样品要求:血浆、血清和其他生物样品,如组织样品。

特异性:亚硝基化蛋白加合物

灵敏度:2 nM

储存和稳定性:在指定温度下储存,自生产日期起 1 年


Kit Contents:

Precoted Microwell strips and strip frames

Wash Buffer

Dilution Buffer

Nitrotyrosine Standard

Biotinylated anti nitrotyrosine tracer

Streptavidin Peroxidase

TMB Substrate

Stop Solution


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