NWLSS™ 人硫氧还蛋白1（Trx1）ELISA试剂盒用于体外定量测定细胞裂解液和缓冲溶液中的人Trx1。该测定将识别天然和重组人Trx1。

硫氧还蛋白(Trx)是一种多功能蛋白，具有抗炎和抗凋亡作用以及抗氧化作用。 因此，Trx是一种公认的涉及氧化应激的各种疾病的生物标志物，是该领域研究的有用生物标志物。

Thioredoxins(Trx) are small, multi-functional proteins with oxido-reductase activity and are ubiquitous in essentially all living cells. Trx contains a redox-active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. The two cysteine residues in the conserved active centers can be oxidized to form intramolecular disulfide bonds. Reduction of the active site disulfide in oxidized Trx is catalyzed by Thioredoxin reductase (TR) with NADPH as the electron donor. The reduced Trx is a hydrogen donor for ribonucleotide reductase, the essential enzyme for DNA synthesis, and a potent general protein disulfide reductase with numerous functions in growth and redox regulations. Specific protein disulfide targets for reduction by Trx include protein disulfide-isomerase (PDI) and a number of transcription factors such as p53, NF-kB and AP-1 (T1-151). Trx is also capable of removing H2O2, particularly when it is coupled with either methionine sulfoxide reductase or several isoforms of peroxiredoxins. To summarize, Trx is a multifunctional protein with anti-inflammatory and antiapoptotic effects, as well as antioxidative effects. Accordingly, Trx is a well-recognized biomarker of various diseases involving oxidative stress and is a useful biomarker for research focused in this area.

格式：96孔板

用途：生物样品中人Trx1的定量测量。

样品：可能存在硫氧还蛋白1的人血浆和其他生物体液

特异性：人硫氧还蛋白-I

灵敏度：0.4 ng/mL 至 25 ng/mL

储存和稳定性：在指定温度下储存，自生产日期起1年

Kit Contents:

Microwell strips precoated w/anti huTrx1 and strip frame

rHu-Thioredoxin 1 Standard (lyophilized)

Sample Dilution Buffer

100X Secondary Antibody (biotin labeled anti-huTrx1)

Reagent Dilution Buffer

100X Avidin-HRP Conjugate

Assay Preparation Buffer

TMB Substrate

Stop Solution

20X Concentrated Wash Buffer

Adhesive Plate Covers