NWLSS™ Human Thioredoxin Reductase 1 (TrxR1) ELISA Kit

NWLSS™ TrxR1 ELISA试剂盒用于体外定量测定人血浆，细胞裂解物和缓冲溶液中存在的硫氧还蛋白还原酶1 (TrxR1)。该测定可识别天然和重组的人TrxR1。

The mammalian thioredoxin reductases (TrxRs) are a family of selenocysteine containing pyridine nucleotide-disulfide oxido-reductases. Three different TrxR isoenzymes, TrxR1 as cytosolic, TrxR2 as mitochondrial, and TrxR3 as testis-specific thiol regulator are known. All the mammalian TrxRs are homologous to glutathione reductase with respect to primary structure including the conserved redox catalytic site (-Cys-Val-Asn-Val-Gly-Cys-) but distinctively with a C-terminal extension containing a catalytically active penultimate selenocysteine(SeCys) residue in the conserved sequence(-Gly-Cys-SeCys-Gly). TrxR is homodimeric protein in which each monomer includes an FAD prosthetic group, a NADPH binding site and a redox catalytic site. Electrons are transferred from NADPH via FAD and the active-site disulfide to C-terminal SeCys-containing redox center, which then reduces the substrate like thioredoxin. The members of TrxR family are 55-58 kDa in molecular size and composed of three isoforms including cytosolic TrxR1, mitochondrial TrxR2, and TrxR3, also known as Trx and GSSG reductase (TGR). TrxR plays a key role in protection of cells against oxidative stress and redox-regulatory mechanism of transcription factors and various biological phenomena. Many tumor cells have elevated TrxR levels and TrxR has been shown to play a major role in drug resistance. Inhibition of TrxR and its related redox reactions may thus contribute to a successful single, combinatory or adjuvant cancer therapy. A great number of effective natural and synthetic TrxR inhibitors are now available possessing antitumor potential ranging from induction of oxidative stress to cell cycle arrest and apoptosis.

Test Principle

The NWLSS™ Thioredoxin Reductase 1 (TrxR1) ELISA kit is to be used for the in vitro quantitative determination of human TrxR1 in human plasma, cell lysate and buffered solution. The assay will recognize both native and recombinant human TrxR1.

格式：96孔板

用途 生物样品中人TrxR1的定量测量。

样品：可能存在硫氧还蛋白还原酶1的人血浆和其他生物液体

特异性：人TrxR1

灵敏度：0.4 ng/mL

储存和稳定性：在指定温度下储存，自生产日期起1年

Kit Contents:

Microwell strips precoated w/anti huTrxR1 and strip frame

rHu-Thioredoxin Reductase 1 Standard (lyophilized)

Sample Dilution Buffer

100X Secondary Antibody (biotin labeled anti-huTrxR1)

Reagent Dilution Buffer

100X Avidin-HRP Conjugate

Assay Preparation Buffer

TMB Substrate

Stop Solution

10X Concentrated Wash Buffer

Adhesive Plate Covers