ABSBio™对氧磷酶1活性检测试剂盒一种简单,灵敏,一步比色法检测多种样品中对氧磷酶(PON1)活性。
该试剂盒提供足够用于100次96孔板分析的试剂,线性检测活性范围为0.05-50 U/L。可以方便地用于384孔分析和高通量分析。
Serum paraoxonase (PON1) is a calcium-dependent esterase associated exclusively with HDL. PON1 exhibit a range of important activities, including drug metabolism and detoxification of organophosphates such as nerve agents. Serum PON1 activity may also be important in avoidance of organophosphate toxicity in industry. PON1 is a mammalian enzyme with hydrolase activity toward multiple substrates.
The ABSBio™ Paraoxonase 1 Activity Detection Kit provides a simple, sensitive, one-step colorimetric assay to detect Paraoxonase (PON1) activity in a variety of samples. In this assay, PON1 hydrolyzes the specific substrate paraoxon to release the p-nitrophenol. The yellow product absorption at 405 nm is directly proportional to the PON1 activity in the sample. The kit is supplied with sufficient reagents for 100 tests in 96-well plate assay, linear detection activity range of 0.05-50 U/L. It could easily be modified for use in 384-well assay and high-throughput assay.
试剂盒组分
应用:Measurement of paraoxonase activity in various biological samples
样品类型:serum, plasma, cell and tissue culture supernatant, urine, saliva, homogenate cell and tissue samples
检测方法:Absorbance (405 nm)
检测限:0.05--50 U/L
反应时间:30 min
存储:-20°C
保质期:6 months after receipt
运输:Icepacks