皮质醇ELISA试剂盒

DetectX®Cortisol ELISA试剂盒定量检测各种样品中的皮质醇。

检测类型:Competitive ELISA

样本类型:Serum, Plasma, Saliva, Urine, Tissue Culture, Respiratory Vapor, Hair, Fecal Extracts

灵敏度:27.6 pg/mL

适应物种:Cortisol在不同物种间是相同的

检测时间:1.5小时

样品数/板:39 in Duplicate

读数:Colorimetric, 450 nm

DetectX®皮质醇ELISA试剂盒经验证样本类型:干燥的粪便提取物,唾液,尿液,血清,EDTA和肝素血浆及组织培养培养基。

Performance:
The Cortisol ELISA Kit is one of the most sensitive cortisol assays on the market, delivering results in as little as 90 minutes using a standard plate reader. The cortisol assay is compatible with a wide variety of sample types from various species and utilizes as little as 1μL of Serum or Plasma to perform the assay.

检测原理:

The Cortisol ELISA Kit quantitatively measures cortisol in saliva, urine, serum, plasma, dried fecal extracts, and tissue culture media samples. Please read the complete kit insert for more information before performing this assay.

Use our provided cortisol standard to generate a standard curve for the assay. Pipette the standards or diluted samples into a transparent microtiter plate coated with our goat anti-mouse IgG antibody. Add the cortisol peroxidase conjugate and the cortisol monoclonal mouse antibody. The immunological reaction occurs between the anti-cortisol monoclonal antibody, the cortisol antigen in the sample or standard, and the cortisol-peroxidase conjugate. Then incubate the mixture for 1 hour shaking at room temperature. As the cortisol concentration in the sample increases, the bound cortisol-peroxidase conjugate decreases, causing a decrease in signal and vice versa. 

After the 1 hour incubation, wash away the excess cortisol-peroxidase and add the TMB substrate. The TMB substrate reacts with the bound cortisol-peroxidase conjugate generating a signal detected by a plate reader at 450nm. Use the intensity and the standard curve to calculate the cortisol concentration in the samples. 

背景:

Cortisol is the primary glucocorticoid produced and secreted by the adrenal cortex. Often referred to as the “stress hormone,” it affects blood pressure, blood sugar levels, and other actions of stress adaptation. Cortisol is an anti-inflammatory hormone playing roles in hypersensitivity, immunosuppression, and disease resistance. Cortisol also promotes gluconeogenesis, liver glycogen deposition, and the reduction of glucose utilization.

Production of cortisol follows an ACTH-dependent circadian rhythm, with a peak level in the morning and decreasing levels throughout the day. Abnormal cortisol levels are correlated with Prostate Cancer, Depression, Schizophrenia, Cushing’s Syndrome, and Addison’s Disease.

Arbor Assays为临床重要生物分子研发最高质量的检测和免疫分析试剂盒,在生物领域建立健全的免疫检测和酶活性试剂盒以定量生物分子。Arbor Assays检测试剂盒具有高灵敏性,适用多物种及操作步骤简单的特点。

北京中昊新生科技作为美国Arbor Assays中国代理,一直为国内客户提供及时高效的配送和更专业的产品技术支持。为您提供Cortisol ELISA Kit,皮质醇ELISA试剂盒最新现货优惠信息。