The DetectX® Estroone-3-Glucuronide (E1G) ELISA Kits quantitatively measures E1G and its metabolites in a variety of samples.

检测类型: Competitive ELISA
样本类型: Urine, Tissue Culture Media, Fecal Extracts
适用物种: Estrone-3-glucuronide (E1G)在不同物种间相同
检测时间: 2.5小时
样品数/板: 39 in Duplicate
读数: Colorimetric, 450 nm
灵敏度: 7.38 pg/mL


检测原理:
The Estrone-3-Glucuronide (E1G) ELISA Kit quantitatively measures E1G in urine, tissue culture media, and fecal extracts. The Estrone-3-Glucuronide (E1G) ELISA Kit is a competitive ELISA with a run time of 2.5 hours. Please read the complete kit insert for more information before performing this assay.

Use our provided E1G standard to generate a standard curve for the assay. Pipette the standards or diluted samples into a transparent microtiter plate coated with our goat anti-rabbit IgG antibody. Add the E1G peroxidase conjugate and the E1G polyclonal rabbit antibody. Then incubate the mixture covered at room temperature, shaking for 2 hours. The immunological reaction occurs between the anti-E1G antibody, the E1G antigen in the sample or standard, and the E1G-peroxidase conjugate. As the E1G concentration in the sample increases, the bound E1G-peroxidase conjugate decreases, causing a decrease in signal and vice versa.

After the 2-hour incubation, wash away the excess E1G-peroxidase conjugate and add the TMB substrate. The TMB substrate reacts with the bound E1G-peroxidase conjugate generating a signal detected by a plate reader at 450nm. Use the intensity and the standard curve to calculate the E1G concentration in the samples.

背景:
Estrone-3-glucuronide (1,3,5(10)-estratrien-3-ol-17-one glucosiduronate, E1G) is the principal secreted form of circulating estradiol in mammals. Ovulation is the critical event of each menstrual cycle that occurs during the reproductive life of healthy females. The three phases of the menstrual cycle are:

The initial phase of the menstrual cycle is where there is only a low risk that would enable viable spermatozoa to survive and reach the ovum.
The fertile period is a phase when the chance of fertilization is at a maximum.
A time of absolute infertility when the ovum is no longer viable. Fertilization of the ovum can only happen during the short period of viability.
Clinical studies have indicated the utility of measuring estrone-3-glucuronide (E1G) and pregnanediol-3-glucuronide (PDG) in samples of urine or fecal extracts to monitor ovarian function in females.