甲状腺素ELISA试剂盒
适应多物种

DetectX®Thyroxine ELISA试剂盒定量检测血清,EDTA血浆,组织培养基样品和粪便提取物中T4含量。

检测类型:

Competitive ELISA

样本类型:

Serum, Plasma, Urine, Fecal Extracts, Tissue Culture Media

灵敏度:

0.23 ng/mL

适应物种:

Thyroxine在不同物种间是相同的

检测时间:

1.5小时

样品数/板:

40 in Duplicate

读数:

Colorimetric, 450 nm

DetectX®甲状腺素ELISA试剂盒试剂盒提供甲状腺素标准品以生成测定的标准曲线，并且应从标准曲线中读取所有样品。将标准品或稀释的样品吸移到涂有抗体的微孔板中，以捕获小鼠抗体。将甲状腺素-过氧化物酶结合物添加到孔中，通过向每个孔中添加针对T4的单克隆抗体来启动结合反应。温育一小时后，洗涤板并添加提供的底物。底物与结合的T4-过氧化物酶缀合物反应。孵育30分钟后，停止反应，并在能够测量450nm波长的微量滴定板读数器中检测所产生颜色的强度。使用酶标仪对样品的稀释液进行适当校正后，可以计算出样品中T4的浓度。

The thyroid hormones, triiodothyronine (T3) and thyroxine (T4) are tyrosine-based hormones produced by the thyroid gland that are primarily responsible for regulation of metabolism. Iodine is necessary for the production of T3 and T4. A deficiency of iodine leads to decreased production of T3 and T4, enlarges the thyroid tissue and will cause the disease known as goitre. The major form of thyroid hormone in blood is T4, which has a longer half-life than T3. The ratio of T4 to T3 released into the blood is roughly 20 to 1. T4 is converted to the active T3 (three to four times more potent than T4) within cells by deiodinases. All three deiodinase isoforms are selenium-containing enzymes, thus dietary selenium is essential for T3 production.

Hypothyroidism is the condition that results from under-production of thyroxine by the thyroid gland either because the gland is naturally underactive or because radioiodine therapy or surgery for an overactive gland has resulted in underactivity. Thyroxine is commonly taken to replace the deficiency which exists in such situations and therefore to restore normal metabolic activity. The concentration of T3 and T4 in the blood regulates the pituitary release of thyrotropin in a negative feedback loop such that when T3 and T4 concentrations are high, TSH production is reduced.

Arbor Assays为临床重要生物分子研发最高质量的检测和免疫分析试剂盒,在生物领域建立健全的免疫检测和酶活性试剂盒以定量生物分子。Arbor Assays检测试剂盒具有高灵敏性,适用多物种及操作步骤简单的特点。

北京中昊新生科技作为美国Arbor Assays中国代理,一直为国内客户提供及时高效的配送和更专业的产品技术支持。为您提供Thyroxine ELISA Kit,甲状腺素ELISA试剂盒最新现货优惠信息。