2',3'-cGAMP检测试剂盒, 2',3'-Cyclic GAMP检测试剂盒

适用多种物种

DetectX® 2’,3’-cGAMP STING-Based FRET试剂盒用于定量检测细胞裂解液和组织以及组织培养基样品中的2’,3’-cGAMP。

检测类型:

Detection Kit

样本类型:

Cell Lysates, Tissue Extracts, Tissue Culture Media

灵敏度:

82.02 pmol/mL

适应物种:

2',3'-Cyclic GAMP在不同物种间是相同的

样品数/板:

40 in duplicate

检测时间:

15分钟

读数:

Fluorescence, 458 nm Excitation and 600 nm/490 nm Emission

STING被用作Pollock等人开发的用于体内和体外测试的生物传感器的基础。选择小鼠STING是因为与人类STING相比，小鼠STING更倾向于与细菌和真核CDN的结合，具有更广泛的应用前景。该STING生物传感器(BioSTING)是一种基于荧光共振能量转移(FRET)的传感器，设计用于实时检测活细胞内的2’,3’-cGAMP。FRET是一种基于两个荧光团之间距离依赖关系的荧光检测平台。

在BioSTING的情况下，mTFP和mKO2是首选的荧光团。当mTFP以458 nm的波长激发、没有配体(2’,3’-cGAMP)存在时，在490 nm处检测到发射。当BioSTING与配体结合时，两个荧光团彼此更靠近，从而使荧光从mTFP转移到mKO2，将发射波长更改为600 nm。利用这种检测方法，BioSTING显示适用于CDN生产调控的体外高通量筛选，以及直接筛选STING激动剂和拮抗剂。

STING was used as the base for a biosensor developed by Pollock et al. for in vivo and in vitro testing. Murine STING was chosen as it has demonstrated a higher propensity to bind to bacterial and eukaryotic CDN’s versus human STING, lending to potentially broader application. This STING biosensor (BioSTING) is a fluorescence resonance energy transfer (FRET) based sensor designed to detect 2’,3’-cGAMP in real-time within living cells. FRET is a fluorescence detection platform that is based on the distance dependent relationship between two fluorophores. In the case of BioSTING, mTFP and mKO2 are the fluorophores of choice. When mTFP is excited with a wavelength of 458 nm, the emission is detected at 490 nm when no ligand (2’,3’-cGAMP) is present. When BioSTING binds to the ligand, the two fluorophores are positioned in closer proximity to each other, allowing the fluorescence to be transferred from mTFP to mKO2, changing the emission wavelength to 600 nm. Using this detection method, BioSTING shows applicability for in vitro high-throughput screening for CDN production modulation and direct screening for STING agonists and antagonists.

Arbor Assays为临床重要生物分子研发最高质量的检测和免疫分析试剂盒,在生物领域建立健全的免疫检测和酶活性试剂盒以定量生物分子。Arbor Assays检测试剂盒具有高灵敏性,适用多物种及操作步骤简单的特点。

北京中昊新生科技作为美国Arbor Assays中国代理,一直为国内客户提供及时高效的配送和更专业的产品技术支持。为您提供2',3'-cGAMP检测试剂盒, 2',3'-Cyclic GAMP检测试剂盒最新现货优惠信息。