ABSbio™ 人IFN-γ ELISA试剂盒/人干扰素Gamma ELISA试剂盒用于定量检测天然和重组人IFN-γ,该试剂盒足以在大约96 ELISA板孔中检测人IFN-γ。
适用样品:血清,血浆和细胞培养基
检测范围:32-2000 pg/ml
别名:IFN-G; IFG; IFI; INFr; IFN, Immune Interferon; Interferon Gamma; 干扰素γ
Interferon-gamma (IFN-γ) is an inflammatory cytokine that has been implicated in the development of fibrosis in inflamed tissues.
ABSbio™ Human IFN-γ ELISA Kit provide a rapid, sensitive and reliable assay for the determination of Human IFNγ in serum, plasma & cell culture media. The strip plate is coated with the antibody and pre-blocked to provide timesaving for high-throughput users. Human IFN-γ containing samples or standards and a biotinylated detection antibody directed against Human IFN-γ are added subsequently and then followed by washing step. Streptavidin-HRP was added and unbound conjugates were washed away. Finally, TMB was used to visualize HRP enzyme reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human IFN-γ in samples.
The kit is designed to quantitatively measure natural and/or recombinant Human IFN-γ within the range of 32–2000 pg/ml. The kit is sufficient to assay human IFN-γ in approximately 96 ELISA plate wells.