ABSbio™人IGFBP1 ELISA试剂盒用于定量检测天然或重组人IGFBP1。该试剂盒足以在大约96 ELISA板孔中检测人IGFBP1。
适用范围:血清,血浆和细胞培养基
检测范围:8-1500 pg/ml
别名:IBP1; AFBP; IGF-BP25; PP12; Placental Protein 12; Amniotic Fluid Binding Protein; Alpha-Pregnancy-Associated Endometrial Globulin; Growth Hormone Independent-Binding Protein; Insulin Like Growth Factor Binding Protein 1; 胰岛素样生长因子结合蛋白1; 胎盘蛋白12
Insulin-like growth factor-binding protein 1 (IBP-1) also known as placental protein 12 (PP12) is a protein that in humans is encoded by the IGFBP1 gene.
ABSbio™ Human IGFBP1 ELISA Kit provide a rapid, sensitive and reliable assay for the determination of Human IGFBP1 in serum, plasma & cell culture media. The strip plate is coated with the antibody and preblocked to provide timesaving for high-throughput users. Human IGFBP1 containing samples or standards and a biotinylated detection antibody directed against Human IGFBP1 are added subsequently and then followed by washing step. Streptavidin-HRP was added and unbound conjugates were washed away. Finally, TMB was used to visualize HRP enzyme reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human IGFBP1 in samples.
The kit is designed to quantitatively measure natural and/or recombinant Human IGFBP1 within the range of 8–1500 pg/ml. The kit is sufficient to assay Human IGFBP1 in approximately 96 ELISA plate wells.