ABSbio™人IL-1α ELISA试剂盒/人白介素1a ELISA试剂盒用于定量检测天然或重组人IL-1α。该试剂盒足以在96 ELISA板孔中检测人IL-1α。

适用样品：血清，血浆和细胞培养基
检测范围：8 - 2500pg /ml
别名：IL1a; IL1-A; IL-1α; IL1F1; Preinterleukin 1 Alpha; Hematopoietin-1; Pro-Interleukin-1-Alpha; Interleukin-1 Family Member 1; Interleukin 1 Alpha

Interleukin-1 alpha (IL-1α) and interleukin-1 beta (IL-1β) are two biochemically distinct, but distantly related, polypeptidic cytokines that play a key role in inflammation, immunologic reactions, and tissue repair. IL-1α has been implicated in the pathogenesis of infectious, autoimmune and inflammatory diseases. 

ABSbio™ Human IL-1α ELISA Kit provide a rapid, sensitive and reliable assay for the determination of Human IL-1α in serum, plasma & cell culture media. The strip plate is coated with the antibody and pre-blocked to provide timesaving for high-throughput users. Human IL-1α containing samples or standards and a biotinylated detection antibody directed against Human IL-1α are added subsequently and then followed by washing step. Streptavidin-HRP was added and unbound conjugates were washed away. Finally, TMB was used to visualize HRP enzyme reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human IL-1α in samples.

The kit is designed to quantitatively measure natural and/or recombinant Human IL-1α within the range of 8–2500 pg/ml. The kit is sufficient to assay Human IL-1α in approximately 96 ELISA plate wells.