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Human Factor X total antigen assay ELISA kit
货号:HFXKT-TOT | 规格:1 kit | 价格:¥0.00 | 品牌:Molecular Innovations
Human Factor X total antigen assay ELISA kit是由北京中昊新生科技有限公司提供,Molecular Innovations专注于凝血和溶栓试剂,包括PAI-827、tPA、尿激酶、凝血因子、玻连蛋白、纤维蛋白原、纤连蛋白、肾素原/肾素、前激肽释放酶、激肽释放酶、白蛋白、纤溶酶原、纤溶酶、抗纤溶酶、凝血酶、抗凝血酶、补体成分和免疫球蛋白。
Factor X is a disulfide linked two-chain glycoprotein zymogen and is the precursor of the coagulation enzyme Factor Xa. Factor X is activated by Factor IXa in complex with Factor VIII, calcium and phospholipids during the intrinsic pathway and by Factor VIIa in complex with Tissue Factor, calcium and phospholipids during the extrinsic pathway of the coagulation cascade. The sensitive quantitative measurement of total human Factor X antigen in plasma samples is easily performed with this 96 well strip format ELISA kit. Factor X, Xa, and Xa in complex with inhibitors or cofactors will be detected by the assay. The concentration of Factor X in normal human plasma is 7-8 ug/ml. The assay measures total human Factor X in the 0.1-50 ng/ml range. Samples giving human Factor X levels above 50 ng/ml should be diluted in blocking buffer before use. A 1:1,000 dilution for plasma is suggested for best results. Human Factor X will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, anti-human Factor X primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with the secondary antibody conjugated to HRP. Following an additional washing step, TMB substrate is used for color development at 450nm. Color development is proportional to the concentration of Factor X in the samples. A standard calibration curve is prepared using dilutions of purified Factor X and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.
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