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Human Immunoglobulin M ELISA Kit
货号:HUIGMKT | 规格:1 kit | 价格:¥0.00 | 品牌:Molecular Innovations
Human Immunoglobulin M ELISA Kit是由北京中昊新生科技有限公司提供,Molecular Innovations专注于凝血和溶栓试剂,包括PAI-1059、tPA、尿激酶、凝血因子、玻连蛋白、纤维蛋白原、纤连蛋白、肾素原/肾素、前激肽释放酶、激肽释放酶、白蛋白、纤溶酶原、纤溶酶、抗纤溶酶、凝血酶、抗凝血酶、补体成分和免疫球蛋白。
Immunoglobulin M (IgM) is the first immunoglobulin produced in the immune response and is the third most abundant immunoglobulin in serum. IgM is a disulfide-linked 970kDa pentamer that activates complement and is responsible for red blood cell agglutination. Each monomer consists of two mu heavy chains and two kappa or lambda light chains. The sensitive quantitative measurement of total human IgM antigen in serum, plasma, hybridoma cell supernatants, ascites or other biological fluid samples is easily performed with this 96 well strip format ELISA kit. The concentration of IgM in normal human serum ranges from 0.5 to 2.0 mg/ml. The assay measures human IgM in the 0.2-200 ng/ml range. Samples giving human IgM levels above 200 ng/ml should be diluted in blocking buffer before use. A 1:25,000 to 1:200,000 dilution for normal human serum or plasma is suggested for best results. Human IgM will bind to the affinity purified capture antibody coated on the microtiter plate. After appropriate washing steps, peroxidase labeled anti-human IgM primary antibody binds to the captured protein. Excess antibody is washed away and TMB substrate is used for color development at 450nm. Color development is proportional to the concentration of human IgM in the samples. A standard calibration curve is prepared in blocking buffer using dilutions of purified human IgM and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.
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