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Mouse tPA total antigen assay ELISA kit
货号:MTPAKT-TOT | 规格:1 kit | 价格:¥0.00 | 品牌:Molecular Innovations
Mouse tPA total antigen assay ELISA kit是由北京中昊新生科技有限公司提供,Molecular Innovations专注于凝血和溶栓试剂,包括PAI-1413、tPA、尿激酶、凝血因子、玻连蛋白、纤维蛋白原、纤连蛋白、肾素原/肾素、前激肽释放酶、激肽释放酶、白蛋白、纤溶酶原、纤溶酶、抗纤溶酶、凝血酶、抗凝血酶、补体成分和免疫球蛋白。
Tissue plasminogen activator (tPA) is a serine protease that converts plasminogen to plasmin in the blood fibrinolytic system. It also plays an important role in the nervous system, including the processes of neuronal migration, neurite outgrowth, and neuronal plasticity. tPA has been suggested to have a role in several neuropathological conditions such as cerebral ischemia, seizures, and demyelinating diseases. The sensitive quantitative measurement of total mouse tPA antigen in plasma and other biological fluid samples is easily performed with this 96 well strip format ELISA kit. The level of tPA antigen in normal plasma was found to be 9.4 ng/ml in NSA/CF-1, 2.4 ng/ml in C57BL6, and 0.4 ng/ml in CD-1 mouse strains. The assay measures total tPA in the 0.1-50 ng/ml range. Samples giving mouse tPA levels above 50 ng/ml should be diluted in blocking buffer before use. Mouse tPA will bind to the capture antibody coated on the microtiter plate. Free and complexed tPA will be detected by the assay. After appropriate washing steps, anti-mouse tPA primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with the secondary antibody conjugated to HRP. Following an additional washing step, TMB substrate is used for color development at 450 nm. Color development is proportional to the concentration of mouse tPA in the samples. A standard calibration curve is prepared in blocking buffer using dilutions of purified mouse tPA and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.
Suggested additional reagents: 10X Wash Buffer, TMB Substrate, tPA Antigen Capture Plate, Secondary Antibody
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