Rat Factor XII total antigen assay ELISA kit
货号:RFXIIKT-TOT
|
规格:1 kit
|
价格:¥0.00
|
品牌:Molecular Innovations
Rat Factor XII total antigen assay ELISA kit是由北京中昊新生科技有限公司提供,Molecular Innovations专注于凝血和溶栓试剂,包括PAI-1711、tPA、尿激酶、凝血因子、玻连蛋白、纤维蛋白原、纤连蛋白、肾素原/肾素、前激肽释放酶、激肽释放酶、白蛋白、纤溶酶原、纤溶酶、抗纤溶酶、凝血酶、抗凝血酶、补体成分和免疫球蛋白。
Factor XII (aka Hageman Factor) is a single-chain, 615 amino acid glycoprotein zymogen. Factor XII is activated by kallikrein. Factor XIIa converts prekallikrein to kallikrein during the intrinsic pathway of the coagulation cascade. Although Factor XII is not thought to play an essential role in normal hemostasis, lack of Factor XII in a mouse model resulted in a severe defect in thrombus formation. The sensitive quantitative measurement of total rat Factor XII antigen in plasma samples is easily performed with this 96 well strip format ELISA kit. Factor XII and XIIa will be detected by the assay. The concentration of Factor XII in normal human plasma ranges from 15-47 ug/ml. Normal values of Factor XII in rat plasma have not been conclusively determined but are believed to be similar to human plasma based on in-house testing. The assay measures total rat Factor XII in the 0.01-10 ng/ml range. Samples giving rat Factor XII levels above 10 ng/ml should be diluted in blocking buffer before use. A 1:10,000 to 1:100,000 dilution for plasma is suggested for best results. Rat Factor XII will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, biotin labeled anti-rat Factor XII primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with streptavidin conjugated to HRP. Following an additional washing step, TMB substrate is used for color development at 450nm. Color development is proportional to the concentration of Factor XII in the samples. A standard calibration curve is prepared using dilutions of purified Factor XII and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.
相关产品
-
货号:NTCYSPAI-L
|
规格:1.0 mg
|
价格:¥0.00
|
品牌:Molecular Innovations
-
货号:PK20E7-BIO
|
规格:1.0 mg
|
价格:¥0.00
|
品牌:Molecular Innovations
-
货号:RbUPA
|
规格:0.05 mg
|
价格:¥0.00
|
品牌:Molecular Innovations
-
货号:FXII15G5
|
规格:0.1 mg
|
价格:¥0.00
|
品牌:Molecular Innovations
-
货号:HPREN-HIS
|
规格:0.1 mg
|
价格:¥0.00
|
品牌:Molecular Innovations
-
货号:FVII11G42D8-BIO
|
规格:10 mg
|
价格:¥0.00
|
品牌:Molecular Innovations
-
货号:PPACK
|
规格:5.0 mg
|
价格:¥0.00
|
品牌:Molecular Innovations
-
货号:HA1AC
|
规格:1.0 mg
|
价格:¥0.00
|
品牌:Molecular Innovations