SARS-CoV-2 surrogate Virus Neutralizing Test (sVNT) (spike protein/ ACE2 ligand binding assay) COVID-19 sVNT
适用物种:human

样本类型:Serum,Plasma

检测范围:N/Ap

最低检出限:N/Ap

测定时间:2.5 hours

检测格式:Competitive ELISA

检测原理:
SARS-CoV-2 spike protein ligand binding assay allows rapid quantification of COIVD19 neutralizing antibodies in serum that block the interaction between the viral spike protein and its target the ACE2 receptor.

检测方法:Peroxidase / OD450 

靶抗原:Neutralizing antibodies

应用:Quantification of neutralizing antibodies

包被板类型:Strip

样品体积:40 uL

特异性:SARS-CoV-2 neutralizing antibodies

检测精度(批内差异,批间差异):<10%, <10%

防腐剂:None

试剂盒组分:
Coated microtiter plate, 96 wells
Calibrators (250 uL) - 0%, 20%, 40%, 60%, 80%, 100% inhibition
10X wash buffer - 50ml
Assay buffer - 50ml
1000X detection reagent - 150 ul
TMB - 12ml
TMB stop solution - 12ml
Plate sealers - 3

储存温度和稳定性:Stable at -20°C for 1 year

背景描述:
Neutralizing antibodies develop in some patients to varying degrees following infection with SARS-CoV-2

检测程序:
This is a competitive ELISA that measures COVID19 neutralizing antibodies in human sera and plasma that prevents the binding of viral spike protein to its ACE2 receptor target.

样本收集:
This kit is compatible with EDTA-plasma, heparinplasma and serum samples. Samples can be stored at or below -20°C for up to 1 year.

样品制备:
Samples are used neat.

试剂制备:
Prepare only the appropriate amount of required reagent on the day of use. Store all reagents as per instructions stated on the label.

结果计算:
1. Neutralizing antibodies will vary in terms of concentration and affinity, this assay provides a qualitative readout. As such the user should use the comparable positive controls inhibitory controls when comparing interassay results. The provided controls are tested for comparability between lots and can be traced.
2. The protein titers in the test samples will fall in the range of high, medium, low or negative. We recommend each lab develop their own statistical cutpoint using methodologies as described by G. Shankar, et al. (2008). (Recommendations for the validation of immunoassays used for detection of host antibodies against biotechnology products. J. Pharmaceutical and Biomedical Analysis 48:1267–1281).
3. Any sample undiluted or diluted still reading greater than the highest standard should be diluted appropriately with assay buffer and retested. If the samples have been diluted, the concentration determined from the standard curve must be multiplied by the dilution factor.



AffinityImmuno致力于为生物药物研究提供高品质ELISA试剂盒和单克隆抗体，同时提供定制鸡多克隆抗体的服务。
北京中昊新生科技是AffinityImmuno中国代理,联系我们查询SARS-COV-2中和抗体检测试剂盒(sVNT),SARS-CoV-2 surrogate Virus Neutralization Test Kit最新优惠信息。