Human Neutrophil Elastase, Lyophilized
货号:HNE-L
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规格:0.5 mg
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价格:¥0.00
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品牌:Molecular Innovations
Human Neutrophil Elastase, Lyophilized是由北京中昊新生科技有限公司提供,Molecular Innovations专注于凝血和溶栓试剂,包括PAI-873、tPA、尿激酶、凝血因子、玻连蛋白、纤维蛋白原、纤连蛋白、肾素原/肾素、前激肽释放酶、激肽释放酶、白蛋白、纤溶酶原、纤溶酶、抗纤溶酶、凝血酶、抗凝血酶、补体成分和免疫球蛋白。
Chromatographically purified from leucocytes of purulent human sputum and lyophilized. The lyophilized, salt-free, soluble powder is stable for over a year when stored at 5 C. The protein is greater than 95percent pure by SDS-PAGE and is free of cathepsin G, MPO and lysozyme. 800-900 units per mg of protein on the substrate Suc-Ala-Ala-Ala-pNA. 15000-19000 units per mg protein on the substrate MeO-Suc-Ala-Ala-Pro-Val-pNA. One unit will hydrolyze 1 nanomole of substrate per minute at pH 7.5 and 25 C. The following diluents and concentrations are recommended: 1) 1 mg/ml in 0.05 M Na Acetate pH 5.0 containing 0.1 M NaCl. Stable 7 days at 5 C (in ice). 2) 10 mg/ml in 50percent glycerol 50percent 0.02 M Na Acetate pH 5. Stable at least 60 days at -20 C with 20 cycles between -20 C and 5 C. 3) May be dissolved at 10 mg/ml in 2 mM HoAc and re-lyophilized in smaller quantities. Stability is better in acidic (pH 5.0) than basic buffers. Inactivation may occur at pH 8.0 due to autolysis and/or proteolysis by trypsin such as bovine pancreatic trypsin. Proteolysis can be inhibited by benzamidine. Autolysis and proteolysis can be inhibited by elastatinal. However, elastatinal is also an inhibitor of elastase. Low pH protects the elastase from both autolysis and proteolysis.
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