小清蛋白抗体

The antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat thalamus, cortex, and hippocampus.

The antiserum has been characterized as specific to parvalbumin; please see reference listed below. Recommended primary dilution is 1/5,000 – 1/8,000 in PBS/0.3% Triton X-100 – biotin/avidin-HRP Technique.

照片描述: IHC image of the rat hippocampus staining for parvalbumin. The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:5000 for 48 hours, goat anti-guinea pig secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen.

The second image above entitled “D.” is parvalbumin (RED) interneurons pictured in mouse striatum, GREEN is GFP expressed by medium spiny neurons, provided Alipi Naydenov, Neurobiology and Behavior, Stella Lab University of Washington (see review below).

宿主: Guinea Pig

规格/体积:  100 µL

性状: Lyophilized Whole Serum

反应性: Rat

别名:  Parvalbumin alpha; Pva; Parvalbumin (calcium binding protein); PALB1, PC255L (Millipore Cat #), anti-parvalbumin

Gene Symbol:  Pvalb