DTSSP crosslinker (3,3´-Dithiobis[sulfosuccinimidylpropionate]) is a homobifunctional protein crosslinker that is cell membrane impermeable. DTSSP crosslinking reagent has primary amine-reactive N-hydroxysulfosuccinimide (Sulfo-NHS) esters at both ends of a cleavable, 8-atom (12.0 angstrom) spacer arm. DTSSP contains a reducible disulfide (thiol) bond in the spacer arm that makes it a good choice for various applications. The disulfide bond can be reduced with TCEP-HCl or DTT to reverse the crosslinked proteins.

DTSSP crosslinking reagent is ideal for aqueous conjugation reactions. DTSSP crosslinker can be used to crosslink extracellular and membrane proteins. If intracellular crosslinking is desired, then DSP, the water insoluble analog of DTSSP can be used for your research.

Molecular Weight: 608.51

Spacer Length: 12.0 Å

CAS Number: 81069-02-5

Alternative names: 1-​[3-​[3-​(2,5-​dioxo-​3-​sulfopyrrolidin-​1-​yl)oxy-​3-​oxopropyl]disulfanylpropanoyloxy]-​2,5-​dioxopyrrolidine-​3-​sulfonic acid; Sulfo-DSP; DTBSSP