AlbuSorb™ Albumin Depletion From Serum or Plasma 从血清或血浆样品中去除白蛋白优势:
♦ 去除率高: 可从30mg/ml的白蛋白样品中去除高达90%以上的白蛋白;
♦ 相当于亲和型: 只会去除样品中的白蛋白,与其他的蛋白质几乎没有交叉反应;
♦ 操作程序简单: 只需要简单的离心或过滤,并且能够恢复血清样本;
♦ 采用新的表面处理技术,而不是基于亲和层析法;
♦ 去除条件温和: 能够在温和的去除条件下维持蛋白质的三级结构,同时简单的转移到二级分析;
♦ 保留酶的生物学活性: 白蛋白耗尽滤液能够保留酶以及生物活性;
♦ 应用物种广泛: 可以去除包括人,小鼠,羊,牛,山羊,大鼠和小牛等物种的血清、血浆样品中的白蛋白;
♦ 通过连续流动馏分与LC-MS兼容,应用于基于活性的蛋白质分析和蛋白质组学研究。AlbuSorb™ is an albumin depletion reagent supplied as a kit with necessary buffers. With 25ul of starting sample, the yield of albumin depleted serum protein is 0.1-0.2 milligrams. AlbuSorb™ binds albumin from serum or plasma and is compatible with downstream proteomics methods such as protein array pixelation ,1D and 2D gel electrophoresis, LC/MS, and MALDI-TOF MS. Samples are also prepared for biomarker discovery, toxicological studies for new drugs, enzyme assays, protein profiling using SELDI analysis and cytokines research.
AlbuSorb™ binds to albumin, and serum proteins flow through. Removal of albumin allows enhanced detection of low abundance proteins. AlbuSorb™ comes from a silica-based, separation platform utilizing a new combination of surface microenvironments substituted with low molecular substrates that feature drug-binding motifs. Unlike immuno-affinity, the surfaces utilized are disposable eliminating cycle to cycle variance and cross-contamination.
Biotech Support Group has AlbuVoid™ for albumin depletion plus low abundance serum protein enrichment. Both AlbuSorb™ and AlbuVoid™ are available in AlbuTrial™ kit™. AlbuTrial™ kit™ includes 1 gm of AlbuSorb™ and 5 Preps of AlbuVoid™ with respective buffers.