KinaSorb™ Kinase (& ATP binding proteins) Enrichment Reagent 激酶（与ATP结合蛋白）富集试剂

用于蛋白激酶和其他ATP结合蛋白的抽提富

KinaSorb™ Kinase (& ATP binding proteins) Enrichment Reagent

Non-covalent immobilization of phosphate group with optimal nucleotide orientation & specificity

Enrichment 3-5X Kinase enrichment, protein recoverable, ~200 µg

60 minute, scaleable protocol compatible with functional assays, electrophoresis and LC-MS

Phosphatase activity & cyclic nucleotide phosphodiesterase (PDE) activity not detectable

Improves protein normalization when comparing heterogeneous tissues

On-bead digestion protocols for LC-MS annotation

KinaSorb™ is a new reagent kit used for the enrichment and isolation of kinases. ATP – the common substrate for kinases, is reversibly immobilized with optimal orientation and specificity, to metallic oxide particles, producing a single-use highly efficient enrichment method for kinase & other ATP binding proteins. The standard prep protocol starts with 100 µl of clarified cellular extracts, or approximately 2 mg total cellular extract protein, but the process can be scaled up or down to accommodate different sample volumes and protein concentrations. The kit includes all necessary reagents for immediate use.