产品优势:

• 有很宽的pH范围：跨度是2-10；
• 无非特异性结合位点：被聚合物的涂层消除了非特异性的结合位点；
• 适用于LC和批量处理样本；
• 应用范围广泛：包括固定化酶，生物大分子的分离和纯化，固定蛋白质与配体，单克隆抗体，激素，多肽，半抗原，药物等。

NuGel Related References：

Patents

Monoclonal antibodies directed to the cytotoxic lymphocyte maturation factor European
Patent EP0790255Purification of immunoglobulins using affinity chromatography and peptide US 2006/0153834 A1

Affinity

Chaumet, Alexandre, Sandrine Castella, Laïla Gasmi, Aurélie Fradin, Gilles Clodic, Gérard
Bolbach, Robert Poulhe, Philippe Denoulet, and Jean-Christophe Larcher. "Proteomic analysis of Interleukin
enhancer binding factor 3 (Ilf3) and Nuclear Factor 90 (NF90) interactome." Biochimie (2013).

Dermot Walls, Robert McGrath and Sinéad T.Loughran A Digest of Protein Purification. Methods Molecular Biology. 
Volume 681: 3-23 (2011) Ehrlich, G. K., Michel, H., Chokshi, H. P. and Malick, A. W. Affinity
purification and characterization of an anti-PEG IgM. Journal of Molecular Recognition, 22: 99–103 (2009).
Development of hepatitis B virus capsids into a whole-chain protein antigen display
platform: New particulate Lyme disease vaccines. International Journal of Medical Microbiology Volume 298, Issues 1-2, 3 January 2008,
Pages 135-142

A sensitive and high-throughput assay to detect low-abundance proteins in serum Hongtao
Zhang, Xin Cheng, Mark Richter & Mark I Greene. Nature Medicine 12, 473 - 477 (2006) Transformation of a L-
peptide epitope into a D-peptide analog. Peptides Frontiers of Peptide Science American Peptide Symposia, 2002, Volume 5, Session XI, 769-770

Expression and folding of an antibody fragment selected in vivo for high expression levels in
Escherichia coli cytoplasm. Research in Microbiology Volume 153, Issue 7, September 2002, Pages 469-474

Identification of model peptides as affinity ligands for the purification of humanized
monoclonal antibodies by means of phage display Journal of Biochemical and Biophysical Methods Volume 49, Issues 1-3.2001

George K. Ehrlich, Pascal Bailon, Wolfgang Berthold. Phage Display Technology -
Identification of Peptides as Model Ligands for Affinity Chromatography Affinity Chromatography Methods in Molecular Biology, 2000, Volume 147, 209-220

A Digest of Protein Purification and partial amino acid sequence of a 28 kDa cyclophilin-like
component of the rat liver sigma receptor. Life Sciences , Volume 55, Issue 8, 1994.

Nachman, M., Azad, A. R. M. and Bailon, P. (1992), Efficient recovery of recombinant proteins
using membrane-based immunoaffinity chromatography (MIC). Biotechnology and Bioengineering, 40: 564–571.

Kinetic aspects of membrane-based immunoaffinity chromatography. Journal of
Chromatography A Volume 597, Issues 1-2, 24 April 1992, Pages 167-172

Identification of model peptides as affinity ligands for the purification of humanized
monoclonal antibodies by means of phage display. Methods in Molecular Biology, 2000, Volume 147, 209-220

Membrane-based receptor affinity chromatography. Journal of Chromatography A Volume
597, Issues 1-2, 24 April 1992, Pages 155-166 9th International Symposium on Affinity Chromatography and Biological Recognition

Ion Exchange

Levin W Protein Purification of recombinant human secretory phospholipase A2 (group II)
produced in long-term immobilized cell culture. Expr Purif 1992 Feb;3(1):27-35.