QIAamp DSP Circulating NA Kit For concentration and purification of free-circulating DNA and RNA from human plasma samples

For 50 preps: includes QIAamp Mini Columns, Buffers, Carrier RNA, QIAGEN Proteinase K, and Tubes.

Features
Concentrated nucleic acids; high input, low elution volumes
Efficient recovery of DNA and RNA
No organic extraction or ethanol precipitation
Removal of contaminants and inhibitors
For in vitro diagnostic use

Product Details
The QIAamp DSP Circulating NA Kit is for concentration and purification of free-circulating DNA and RNA from plasma for in vitro diagnostic use.

Performance
The QIAamp DSP Circulating NA Kit provides ease-of-use in diagnostic workflows, and is manufactured in accordance with GMP requirements. The procedure isolates and purifies high quality nucleic acids for use in downstream applications. The concentration of free-circulating nucleic acids in human blood plasma is usually low and varies considerably between individuals, ranging from 1–100 ng/ml in human samples. Nucleic acids are efficiently purified and concentrated from starting materials that contain low concentrations of mostly fragmented DNA and RNA (typically 1–100 ng/ml circulating DNA in human plasma).

Principle
The QIAamp DSP Circulating NA Kit simplifies isolation of circulating DNA and RNA from plasma samples by omitting phenol-chloroform extractions. Nucleic Acids bind specifically to the QIAamp Mini columns and contaminants pass through. PCR inhibitors are completely removed in the wash steps, leaving pure nucleic acids for elution with the provided buffer.

The QIAamp DSP Circulating NA Kit combines the selective binding properties of silica-based membrane with flexible elution volumes between 20 µl and 150 µl.

Tube extenders and vacuum processing on the QIAvac 24 Plus enable starting sample volumes of up to 5 ml . Flexible elution volumes between 20 µl and 150 µl allow concentration of nucleic acid species that are present in low concentrations.

Procedure
The QIAamp DSP Circulating NA procedure includes 4 steps (lyse, bind, wash, and elute) that are carried out using QIAamp Mini columns on a vacuum manifold. The simple procedure is highly suited for simultaneous processing of multiple samples. Nucleic acids are efficiently purified and concentrated. Additionally, the QIAamp DSP Circulating NA Kit offers two distinct protocols: the Classic and Breeze protocols). The Classic protocol is for processing up to 5 ml plasma in 1 ml steps, and constitutes the unchanged protocol provided in the in the QIAamp DSP Circulating NA Kit Handbook, Version 1, Revision 3 (R3). The Breeze protocol is for processing up to 5 ml plasma in 1 ml steps, but has been optimized for low hands-on and turnaround time.

Applications
ccfDNA purified using the QIAamp DSP Circulating NA Kit is ready for use in a wide range of downstream applications, including NGS, RT-PCR, digital PCR, multiplex PCR and quantitative PCR.