用于对目标RNA测序所需样本和对QIAseq Targeted RNA Panels生成的RNA文库测序进行索引

Features

为Illumina和Ion Torrent测序仪提供最多96个样本的索引

每个索引可足够用于4份样本

管式包装具有灵活性，板式包装符合便利性和高通量测定的要求

Product Details

QIAseq Targeted Panels enable Sample to Insight next-generation sequencing (NGS) of DNA or RNA. These optimized solutions facilitate ultrasensitive variant and clonotype detection from cells, tissue and biofluids using integrated Unique Molecular Indices (UMIs).

In order to de-multiplex pooled libraries and assign reads to the appropriate original source library, NGS workflows require the addition of an adapter complex that includes both a common nucleotide sequence, as well as sample index oligonucleotides.

Compatible only with Illumina instruments, our Unique Dual Indexes (UDIs) are optimized for QIAseq Targeted Panel workflows, including QIAseq Targeted DNA, QIAseq RNAscan and QIAseq Immune Repertoire RNA Library Kits. Exclusively available from QIAGEN for QIAseq targeted NGS applications, QIAseq UDI Kits provide the highest level of process safety and ensure confidence in sequencing data by mitigating “index hopping” and mis-assignment of reads. The combination of both UMIs and UDIs provide QIAseq Targeted Panels with a remarkable level of accuracy. Combinatorial, non-unique dual index adapters are also available.

Performance

Accuracy: Innovative digital sequencing (incorporating molecular barcodes) eliminates PCR duplication and amplification artifacts to detect low-frequency variants with high confidence .

Specificity: The unique combination of our proprietary primer design algorithm and rigorous testing of every primer assay guarantees high specificity and accurate results.

Uniformity: The QIAseq Targeted DNA Panel workflow has been optimized to deliver highly uniform sequencing results, to ensure sequencing capacity is utilized very efficiently .

Sensitivity: Digital DNA sequencing approach is optimized to deliver high confidence in calling low-frequency DNA variants. Over 90% sensitivity for 1% NA12878 SNP and indel on typical coding region with false positive less than 15 per mega base region when variants are detected with tiled primer design to cover complete coding region of each gene.

Universality: The chemistry used in the QIAseq Targeted DNA Panels and workflow is compatible with both regular and GC-rich genomic regions, allowing one to achieve 100% coverage of genes rich in GC content such as CEBPA and CCND1 .

Flexibility: The QIAseq Targeted DNA Panels offer a high degree of flexibility in content and sample multiplexing. Several cataloged panels have been developed for a wide range of applications. One can also build a custom panel for a specific content, or extend the contents of an existing cataloged panel. Up to 384 samples can be multiplexed using the QIAseq indices.

Principle

PCR duplicates are a major issue in targeted DNA sequencing, since, through PCR amplification, they turn unique DNA molecules into identical DNA molecules that cannot be distinguished from each other. In addition, errors from PCR amplification and sequencing process may also be present in final reads that lead to false-positive variants in sequencing results. This, in turn, results in the inability to confidently call DNA variants present at low frequencies in the starting DNA material. To overcome the issue of PCR duplicates and amplification artifacts, the QIAseq Targeted DNA Panels use digital sequencing by incorporating molecular barcodes into the starting DNA material before any amplification takes place, thereby preserving the uniqueness of the starting DNA molecules and overcoming the issues of PCR duplicates, false positives and library bias.

Procedure

The entire workflow of the QIAseq Targeted DNA Panels to go from extracted DNA to sequencing-ready libraries can be completed in 9 hours. Extracted DNA is fragmented, genomic targets are molecularly barcoded and enriched, and libraries are constructed. Sequencing files can be fed into the QIAseq pipeline, a cloud-based data analysis pipeline, which will filter, map and align reads, as well as count unique molecular barcodes associated with targeted genomic regions, and call variants with a barcode-aware algorithm. This data can then be fed into IVA or QCI for interpretation.

Applications

生物标志物研究

全转录组测序数据验证

微阵列数据验证